Chang Dong Woo, Kim Bong Kyung, Shin Jae Hoon, Yoon Young Min, Oh Seung Hyun, Yoon Yeo Sung, Hong Sung-Hyeok, Lee Ki Chang, Lee Young Won, Seo Kang Moon, Kweon Oh-Kyeong, Yoon Jung Hee, Shin Nam-Shik, Lee Kyu Ho, Suh Jun-Gyo, Seong Je Kyung
Department of Veterinary Radiology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Anat Cell Biol. 2010 Jun;43(2):118-24. doi: 10.5115/acb.2010.43.2.118. Epub 2010 Jun 30.
In order to study the treatment of aneurysms, the technique of making experimental aneurysms in laboratory animals must be established. In our study, to examine the feasibility of making experimental aneurysm and selective angiography on the common carotid artery in rabbits and to determine the size of experimental aneurysm after surgery, saccular aneurysms were fashioned on the right common carotid artery in 17 rabbits using a vein pouch technique. Selective angiography of the common carotid artery was performed immediately after surgery, and at 1 week, 4 weeks, and 8 weeks after surgery. Also, histological changes in the aneurysms were observed. In 16 rabbits with established successful experimental aneurysm, no differences were found in diet intake and behavior before and after surgery. The patency of the carotid artery was confirmed by selective angiography. The average size of the aneurysm immediately after surgery was similar to that of 1 week postoperatively in selective angiography, however it increased with time at 4weeks and 8 weeks. Histologically, infiltration of inflammatory cells and hemorrhage were found at the junction of the carotid artery and the vein pouch at 1 week, which disappeared at 4 weeks and 8 weeks. This study suggests experimental saccular aneurysm using the vein pouch technique might form aneurysms similar to that of the human in its properties such as increment of size, and selective angiography might be suitable for assessment of experimental aneurysm. Therefore, this animal model may be suitable for investigating new treatment methodologies for human aneurysms.
为了研究动脉瘤的治疗方法，必须建立在实验动物身上制造实验性动脉瘤的技术。在我们的研究中，为了检验在兔颈总动脉制造实验性动脉瘤和进行选择性血管造影的可行性，并确定手术后实验性动脉瘤的大小，采用静脉袋技术在17只兔的右侧颈总动脉上制作了囊状动脉瘤。术后立即以及术后1周、4周和8周对颈总动脉进行选择性血管造影。同时，观察动脉瘤的组织学变化。在16只成功建立实验性动脉瘤的兔中，手术前后的饮食摄入和行为没有差异。通过选择性血管造影证实了颈动脉的通畅性。手术刚结束时动脉瘤的平均大小与术后1周选择性血管造影时相似，但在4周和8周时随时间增加。组织学上，术后1周在颈动脉与静脉袋交界处发现炎性细胞浸润和出血，4周和8周时消失。本研究表明，采用静脉袋技术制作的实验性囊状动脉瘤在大小增加等特性方面可能形成与人动脉瘤相似的动脉瘤，选择性血管造影可能适用于评估实验性动脉瘤。因此，这种动物模型可能适合于研究人类动脉瘤的新治疗方法。
