Experimental Medicine Laboratory, Universidade Federal de Sergipe-UFS, Rua Manoel Domingos Pereira, 378, Itabaiana, Sergipe 49500-000, Brazil.
Neuroradiology. 2011 Nov;53(11):875-81. doi: 10.1007/s00234-010-0785-3. Epub 2010 Nov 23.
Experimental saccular aneurysms can be created with surgical techniques of the arterial wall or by injecting pancreatic elastase. Papain is an enzyme with properties similar to those of elastase, and it has not been tested for this purpose. The objective of this study was to determine whether papain produces saccular aneurysms.
Eleven New Zealand white rabbits (1.9-3.0 kg) were divided into two groups: group I (n = 8)-papain, and group II (n = 3)-sham. The animals underwent surgical exposure of the neck; the right common carotid artery was used as the test and the left common carotid artery as the control. On the 21st day after surgery, animals were sacrificed for removal of the arteries, measurements, and histological analysis. We determine formation of aneurysm to occur when the test artery dilated compared to the control.
There was no aneurysm formation in the sham group. The papain group showed aneurysm formation in all cases (100%). The average diameter of the aneurysms was 3.8 ± 1.4 mm and the average length was 16.7 ± 6.0 mm. The histological analysis showed a destruction of the elastic fibers in 100% of cases, mild inflammation in 62.5%, intimal fibrosis in 50%, endothelial injury in 100%, and thrombosis in 100% of cases.
Papain was capable of forming aneurysms with histological characteristics similar to those of elastase-induced aneurysms; however, a comparative study is necessary to determine whether the papain is superior to elastase in the production of experimental saccular aneurysms.
实验性囊状动脉瘤可通过动脉壁的外科技术或注射胰腺弹性酶来创建。木瓜蛋白酶是一种具有与弹性酶相似特性的酶，尚未对此进行过测试。本研究的目的是确定木瓜蛋白酶是否会产生囊状动脉瘤。
将 11 只新西兰白兔（1.9-3.0 公斤）分为两组：I 组（n=8）-木瓜蛋白酶，和 II 组（n=3）-假手术。动物接受颈部手术暴露；右颈总动脉用作测试，左颈总动脉用作对照。手术后第 21 天，处死动物以取出动脉、测量和组织学分析。我们确定当测试动脉与对照动脉相比扩张时，动脉瘤形成。
假手术组无动脉瘤形成。木瓜蛋白酶组所有病例均出现动脉瘤形成（100%）。动脉瘤的平均直径为 3.8±1.4 毫米，平均长度为 16.7±6.0 毫米。组织学分析显示 100%的病例弹性纤维破坏，62.5%的病例轻度炎症，50%的病例内膜纤维化，100%的病例内皮损伤，100%的病例血栓形成。
木瓜蛋白酶能够形成具有与弹性酶诱导的动脉瘤相似组织学特征的动脉瘤；然而，需要进行比较研究以确定木瓜蛋白酶在产生实验性囊状动脉瘤方面是否优于弹性酶。
