Hammad F T, Lubbad L
Department of Surgery, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al Ain, United Arab Emirates.
Urol Res. 2011 Oct;39(5):351-6. doi: 10.1007/s00240-010-0348-4. Epub 2010 Dec 29.
The objective of the study was to investigate the effect of diclofenac sodium, when administered similar to its use in clinical practice, 2 weeks following different periods of complete reversible unilateral ureteric obstruction in the rat using accurate indicators of renal function. Male Wistar rats underwent reversible left ureteric obstruction for 2 days [D2-untreated (n = 8) and D2-DS (n = 8) groups] and 5 days [D5-untreated (n = 7) and D5-DS (n = 7) groups]. D2-DS and D5-DS received intramuscular diclofenac sodium (3 mg/kg per day) during the time of obstruction, whereas D2-untreated and D5-untreated groups did not receive any treatment. The renal functions of both kidneys were studied 2 weeks following the reversal. The basal heart rate and mean arterial blood pressure were similar in D2-untreated and D2-DS and in D5-untreated and D5-DS groups. In the D2-untreated group, the GFR and RBF of the left obstructed kidney were 76 and 72% that of the right non-obstructed kidney [0.99 ± 0.06 vs. 1.30 ± 0.08 (P < 0.05) and 4.25 ± 0.33 vs. 5.92 ± 0.61 (P < 0.05), respectively]. The urine volume and urinary and fractional sodium excretion were not significantly different from the right kidney. Similar response was obtained in the D2-DS group. In the D5-untreated group, the GFR and RBF of the left kidney was 66 and 62% that of the right kidney (0.80 ± 0.12 vs. 1.21 ± 0.6, P < 0.05 and 3.79 ± 0.32 vs. 6.16 ± 0.59, P < 0.05; respectively). The tubular functions of both kidneys were similar. In the D5-DS group, there was no difference in GFR or RBF between both kidneys (1.02 ± 0.08 vs. 1.12 ± 0.3, P = 0.24 and 5.10 ± 1.25 vs. 6.46 ± 1.11, P = 0.44, respectively). Similarly, there was no difference in the tubular functions. Treatment with diclofenac sodium during a relatively long period of reversible unilateral ureteric obstruction, similar to its use in the management of ureteric colic, appears to ameliorate the alterations in the hemodynamic glomerular functions at least 2 weeks following the reversal of obstruction.
本研究的目的是，在大鼠经历不同时长的完全可逆性单侧输尿管梗阻后2周，使用准确的肾功能指标，研究双氯芬酸钠在按照其临床实际使用方式给药时的效果。雄性Wistar大鼠经历了2天[D2-未治疗组（n = 8）和D2-DS组（n = 8）]和5天[D5-未治疗组（n = 7）和D5-DS组（n = 7）]的可逆性左输尿管梗阻。D2-DS组和D5-DS组在梗阻期间接受肌肉注射双氯芬酸钠（每天3 mg/kg），而D2-未治疗组和D5-未治疗组未接受任何治疗。梗阻解除后2周研究双侧肾脏的肾功能。D2-未治疗组与D2-DS组以及D5-未治疗组与D5-DS组的基础心率和平均动脉血压相似。在D2-未治疗组中，左梗阻肾脏的肾小球滤过率（GFR）和肾血流量（RBF）分别为右非梗阻肾脏的76%和72%[分别为0.99±0.06对1.30±0.08（P<0.0５）和4.25±0.33对5.92±0.61（P<0.05）]。尿量、尿钠排泄量和尿钠排泄分数与右肾无显著差异。D2-DS组获得了类似的结果。在D5-未治疗组中，左肾的GFR和RBF分别为右肾的66%和62%（0.80±0.12对1.21±0.6，P<0.05；3.79±0.32对6.16±0.59，P<0.05）。双侧肾脏的肾小管功能相似。在D5-DS组中，双侧肾脏的GFR或RBF无差异（分别为1.02±0.08对1.12±0.3，P = 0.24；5.10±1.25对6.46±1.11，P = 0.44）。同样，肾小管功能也无差异。在相对较长时间的可逆性单侧输尿管梗阻期间，按照其在输尿管绞痛治疗中的使用方式给予双氯芬酸钠治疗，似乎在梗阻解除后至少2周可改善血流动力学肾小球功能的改变。
