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SAGA: rapid automatic mainchain NMR assignment for large proteins.SAGA：用于大型蛋白质的快速自动主链 NMR 分配。
J Biomol NMR. 2010 Apr;46(4):281-98. doi: 10.1007/s10858-010-9403-2. Epub 2010 Mar 16.
2
Heme regulatory motifs in heme oxygenase-2 form a thiol/disulfide redox switch that responds to the cellular redox state.血红素加氧酶-2中的血红素调节基序形成一个硫醇/二硫键氧化还原开关，该开关对细胞氧化还原状态作出反应。
J Biol Chem. 2009 Jul 31;284(31):20556-61. doi: 10.1074/jbc.M109.015651. Epub 2009 May 27.
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Sequence-specific resonance assignment of soluble nonglobular proteins by 7D APSY-NMR spectroscopy.通过7D APSY-NMR光谱法对可溶性非球状蛋白质进行序列特异性共振归属
J Am Chem Soc. 2007 Sep 5;129(35):10823-8. doi: 10.1021/ja072564+. Epub 2007 Aug 11.
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Automated resonance assignment of proteins: 6D APSY-NMR.蛋白质的自动共振归属：6D APSY-NMR
J Biomol NMR. 2006 May;35(1):27-37. doi: 10.1007/s10858-006-0030-x. Epub 2006 May 31.
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Hyperdimensional NMR spectroscopy.超维核磁共振光谱学。
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Automated projection spectroscopy (APSY).自动投影光谱学（APSY）。
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7
G-matrix Fourier transform NOESY-based protocol for high-quality protein structure determination.基于G矩阵傅里叶变换NOESY的高质量蛋白质结构测定方案
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G-matrix Fourier transform NMR spectroscopy for complete protein resonance assignment.用于完整蛋白质共振归属的G矩阵傅里叶变换核磁共振光谱法。
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Projection-reconstruction technique for speeding up multidimensional NMR spectroscopy.用于加速多维核磁共振光谱的投影重建技术。
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Automatic determination of protein backbone resonance assignments from triple resonance nuclear magnetic resonance data.从三重共振核磁共振数据中自动确定蛋白质主链共振归属
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用于解决无规卷曲蛋白质骨架 assignments 中 HN 重叠的伪 4D 三重共振实验。

Pseudo-4D triple resonance experiments to resolve HN overlap in the backbone assignment of unfolded proteins.

机构信息

Department of Biological Chemistry, University of Michigan Medical School, MSRB III, 1150 W. Medical Center Drive, Ann Arbor, MI 48109-5605, USA.

出版信息

J Biomol NMR. 2011 Feb;49(2):69-74. doi: 10.1007/s10858-010-9465-1. Epub 2010 Dec 30.

DOI:10.1007/s10858-010-9465-1
PMID:21190062
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3091507/
Abstract

The solution NMR resonance assignment of the protein backbone is most commonly carried out using triple resonance experiments that involve (15)N and (1)HN resonances. The assignment becomes problematic when there is resonance overlap of (15)N-(1)HN cross peaks. For such residues, one cannot unambiguously link the "left" side of the NH root to the "right" side, and the residues associated with such overlapping HN resonances remain often unassigned. Here we present a solution to this problem: a hybrid (4d,3d) reduced-dimensionality HN(CO)CA(CON)CA sequence. In this experiment, the Ca(i) resonance is modulated with the frequency of the Ca(i-1) resonance, which helps in resolving the ambiguity involved in connecting the Ca(i) and Ca(i-1) resonances for overlapping NH roots. The experiment has limited sensitivity, and is only suited for small or unfolded proteins. In a companion experiment, (4d,3d) reduced-dimensionality HNCO(N)CA, the Ca(i) resonance is modulated with the frequency of the CO(i-1) resonance, hence resolving the ambiguity existent in pairing up the Ca(i) and CO(i-1) resonances for overlapping NH roots.

摘要

该蛋白质骨架的溶液 NMR 共振分配最常使用涉及 (15)N 和 (1)HN 共振的三重共振实验来进行。当 (15)N-(1)HN 交叉峰存在共振重叠时，分配就会出现问题。对于这样的残基，人们不能将“左侧”的 NH 根与“右侧”明确地联系起来，并且与这些重叠 HN 共振相关的残基通常仍然未被分配。在这里，我们提出了一个解决这个问题的方法：一种混合 (4d,3d) 降维 HN(CO)CA(CON)CA 序列。在这个实验中，Ca(i)共振的频率调制 Ca(i-1)共振，这有助于解决连接重叠 NH 根的 Ca(i)和 Ca(i-1)共振所涉及的歧义。该实验的灵敏度有限，仅适用于小或未折叠的蛋白质。在一个配套的实验中，(4d,3d) 降维 HNCO(N)CA，Ca(i)共振的频率调制 CO(i-1)共振，因此解决了配对重叠 NH 根的 Ca(i)和 CO(i-1)共振的歧义。