Dynasty BioID Inc., Yinchuan Economical & Technological Zone, Yinchuan, Ningxia, PR China.
Forensic Sci Int. 2011 May 20;208(1-3):139-42. doi: 10.1016/j.forsciint.2010.11.017. Epub 2010 Dec 28.
Cashmere is a high-priced commodity in the world market. For financial gains, various interested parties often adulterate cashmere with cheap sheep wool. Here, we describe a method that can quickly extract mitochondrial DNA from natural or processed animal hair. We further designed two sets of TaqMan polymerase chain reaction (PCR) primers and probes that can react specifically to goat and sheep mitochondrial 12S ribosomal RNA (rRNA) genes. Using TaqMan PCR, we can not only distinguish between cashmere and wool but also quantify their contents in a cashmere/wool mixture. The method can be applied directly to examine the quality of cashmere products in the world markets.
开司米是国际市场上的高价商品。为了获取经济利益，各种利益相关方经常将开司米与廉价的绵羊羊毛混合。在这里，我们描述了一种可以从天然或加工过的动物毛发中快速提取线粒体 DNA 的方法。我们进一步设计了两套 TaqMan 聚合酶链反应（PCR）引物和探针，它们可以特异性地反应山羊和绵羊线粒体 12S 核糖体 RNA（rRNA）基因。使用 TaqMan PCR，我们不仅可以区分开司米和羊毛，还可以定量混合在开司米/羊毛中的含量。该方法可直接用于检测国际市场上的开司米产品的质量。
