Kumar Rajiv, Shakyawar D B, Pareek P K, Raja A S M, Prince L L L, Kumar Satish, Naqvi S M K
Animal Biotechnology Section, ICAR-Central Sheep and Wool Research Institute, Avikanagar, CSWRI Campus, Jaipur, Rajasthan, 304501, India,
Appl Biochem Biotechnol. 2015 Apr;175(8):3856-62. doi: 10.1007/s12010-015-1552-z. Epub 2015 Feb 25.
Pashmina fiber is one of major specialty animal fiber in India. The quality of Pashmina obtained from Changthangi and Chegu goats in India is very good. Due to restricted availability and high prices, adulteration of natural prized fibers is becoming a common practice by the manufacturers. Sheep wool is a cheap substitute, which is usually used for adulteration and false declaration of Pashmina-based products. Presently, there is lack of cost-effective and readily available methodology to identify the adulteration of Pashmina products from other similar looking substitutes like sheep wool. Polymerase chain reaction (PCR)-based detection method can be used to identify origin of animal fiber. Extraction of quality DNA from dyed and processed animal fiber and textile materials is a limiting factor in the development of such detection methods. In the present study, quality DNA was extracted from textile materials, and PCR-based technique using mitochondrial gene (12S rRNA) specific primers was developed for detection of the Pashmina in textile blends. This technique has been used for detection of the adulteration of the Pashmina products with sheep wool. The technique can detect adulteration level up to 10 % of sheep/goat fibers in textile blends.
克什米尔羊毛纤维是印度主要的特种动物纤维之一。从印度的昌唐吉山羊和切古山羊身上获取的克什米尔羊毛质量非常好。由于供应有限且价格高昂，制造商对天然珍贵纤维进行掺假已成为一种常见做法。羊毛是一种廉价的替代品，通常被用于克什米尔羊毛制品的掺假和虚假申报。目前，缺乏一种经济高效且易于使用的方法来鉴别克什米尔羊毛制品与其他外观相似的替代品（如羊毛）之间的掺假情况。基于聚合酶链反应（PCR）的检测方法可用于鉴别动物纤维的来源。从染色和加工过的动物纤维及纺织材料中提取高质量的DNA是开发此类检测方法的一个限制因素。在本研究中，从纺织材料中提取了高质量的DNA，并开发了一种基于PCR技术、使用线粒体基因（12S rRNA）特异性引物的方法来检测纺织混纺物中的克什米尔羊毛。该技术已用于检测克什米尔羊毛制品中羊毛的掺假情况。该技术可检测出纺织混纺物中高达10%的绵羊/山羊纤维掺假水平。
