Geng Qing-Rong
a College of Life Science and Technology, Yancheng Teachers University , Yancheng , Jiangsu Province , P.R China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1808-11. doi: 10.3109/19401736.2014.963823. Epub 2014 Sep 26.
In this article attempts were made to establish one-step duplex PCR assay for the identification of goat cashmere and sheep wool. Primers were selected from published papers or designed in the well-conserved region of mitochondrial D-loop genes after alignment of the available sequences in the GenBank database. A fragment of 294 bp from cashmere goat was amplified and three PCR fragments including a bright main band of approximately 404 bp in length were obtained from sheep. The duplex PCR was found to be effective in detecting mixed samples precisely when sheep wool was mixed to goat cashmere with the relative proportion of over 9.09%. The duplex PCR could be considered as a simple and promising method in identification of goat cashmere and sheep wool.
在本文中，我们尝试建立一种用于鉴定山羊绒和绵羊毛的一步法双重PCR检测方法。引物是从已发表的论文中选取，或者是在对GenBank数据库中可用序列进行比对后，在线粒体D-loop基因的保守区域设计的。从绒山羊中扩增出一段294bp的片段，从绵羊中获得了三个PCR片段，其中包括一条明亮的主带，长度约为404bp。当绵羊毛与山羊绒以超过9.09%的相对比例混合时，发现该双重PCR能够精确检测混合样本。双重PCR可被认为是一种鉴定山羊绒和绵羊毛的简单且有前景的方法。
