The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
Bioresour Technol. 2011 Feb;102(4):3958-65. doi: 10.1016/j.biortech.2010.12.031. Epub 2010 Dec 13.
In this study, the pretreatment of cassava residues by thermal-dilute sulfuric acid (TDSA) hydrolysis was investigated by means of a statistically designed set of experiments. A three-factor central composite design (CCD) was employed to identify the optimum pretreatment condition of cassava residues for methane production. The individual and interactive effects of temperature, H(2)SO(4) concentration and reaction time on increase of methane yield (IMY) were evaluated by applying response surface methodology (RSM). After optimization, the resulting optimum pretreatment condition was 157.84°C, utilizing 2.99% (w/w TS) H(2)SO(4) for 20.15 min, where the maximum methane yield (248 mL/g VS) was 56.96% higher than the control (158 mL/g VS), which was very close to the predict value 56.53%. These results indicate the model obtained through RSM analysis is suit to predict the optimum pretreatment condition and there is great potential of using TDSA pretreatment of cassava residues to enhance methane yield.
本研究采用统计设计的实验方案，考察了热稀硫酸（TDSA）水解预处理木薯废渣的效果。采用三因素中心组合设计（CCD）来确定用于甲烷生产的木薯废渣的最佳预处理条件。通过响应面法（RSM）评估温度、H2SO4 浓度和反应时间对甲烷产量增加（IMY）的单独和交互作用的影响。优化后，得到的最佳预处理条件为 157.84°C，使用 2.99%（w/w TS）的 H2SO4 反应 20.15min，最大甲烷产量（248mL/g VS）比对照（158mL/g VS）提高了 56.96%，与预测值 56.53%非常接近。这些结果表明，通过 RSM 分析得到的模型适合预测最佳预处理条件，并且 TDSA 预处理木薯废渣有很大潜力提高甲烷产量。
