Terra de Oliveira Deborah, de Jesus Paiva Rutiléia, Albuquerque de Mescouto Vanessa, Ferreira da Silva Sara Roberta, Farias Da Costa Ana Alice, Santos Agenor Valadares, Gonçalves Evonnildo Costa, Narciso da Rocha Filho Geraldo, Rodrigues Noronha Renata Coelho, Santos do Nascimento Luís Adriano
Amazon Oil Laboratory, Guamá Science and Technology Park, Belém, 66075-750, Brazil.
Graduation Program of Biotechnology, Institute of Biological Sciences, Federal University of Pará, Belém, 66075-110, Brazil.
Heliyon. 2024 Aug 19;10(16):e36343. doi: 10.1016/j.heliyon.2024.e36343. eCollection 2024 Aug 30.
Renewable energy has been recognized as an alternative to fossil fuels as a step to transform the energy produced and consumed worldwide. Cyanobacteria and microalgae are currently being considered as substitutes to the traditional feedstock used to produce biofuels due to their ability to achieve high amounts of lipids under cellular stress conditions. The aim of this study was to investigate the utilization of sp. CACIAM 22 cyanobacterial biomass as a feedstock for biodiesel production, specifically by examining the effects of supplementing with hydrolysate of Brazil nutshell (HBNS) on biomass generation, lipid production, fatty acid composition, and quality of synthesized biodiesel. The supplementation of HBNS led to a significant increase of 12g.L in wet biomass production. The lipid content reached 41 % of the biomass produced in HBNS supplemented cultures when nitrate source was deprived. The quality evaluation of cyanobacteria-derived biodiesel was performed using Biodiesel Analyzer ver 2.2 software, revealing superior quality compared to biodiesel produced from plant sources. The biodiesel exhibited values of 23 h for oxidative stability, 65 for cetane number, and an iodine index of 31 (g I. 100 g ), indicating promising potential as a renewable source. This study is the first to utilize HBNS as an organic supplement for cyanobacteria culture medium and assess its impact on biomass and lipid production in sp., supporting the hypothesis of utilizing this biomass as a renewable feedstock for biodiesel production as a viable alternative to plant sources based on biomass production, lipid productivity, and biodiesel quality.
可再生能源已被视为化石燃料的替代品，是全球能源生产和消费转型的重要一步。由于蓝藻和微藻在细胞应激条件下能够积累大量脂质，目前它们正被视为生产生物燃料的传统原料的替代品。本研究旨在探讨sp. CACIAM 22蓝藻生物质作为生物柴油生产原料的利用情况，具体研究添加巴西坚果壳水解物（HBNS）对生物质生成、脂质产量、脂肪酸组成以及合成生物柴油质量的影响。添加HBNS使湿生物质产量显著增加了12g.L。在缺乏硝酸盐源的情况下，添加HBNS的培养物中脂质含量达到所产生生物质的41%。使用Biodiesel Analyzer ver 2.2软件对蓝藻衍生生物柴油进行质量评估，结果显示其质量优于植物源生物柴油。该生物柴油的氧化稳定性为23小时，十六烷值为65，碘值为31（g I. 100 g），表明其作为可再生能源具有广阔的应用前景。本研究首次将HBNS用作蓝藻培养基的有机补充剂，并评估其对sp.生物质和脂质生产的影响，从生物质产量、脂质生产率和生物柴油质量等方面支持了将这种生物质作为生物柴油生产的可再生原料以替代植物源的假设。
