Sada E, Ferguson L E, Daniel T M
Department of Microbiology, Instituto Nacional de Enfermedades Respiratorias, Mexico, DF.
J Infect Dis. 1990 Oct;162(4):928-31. doi: 10.1093/infdis/162.4.928.
An ELISA was established for the measurement of IgG antibody in human serum to the 30,000-Da native antigen of Mycobacterium tuberculosis and evaluated for its utility in the diagnosis of tuberculosis at the Instituto Nacional de Enfermedades Respiratorias in Mexico City. The test had a sensitivity of 70% in patients with sputum-positive active pulmonary tuberculosis and a specificity in control subjects of 100%. The accuracy of positive prediction was 100% and of negative prediction 93% for patients with pulmonary tuberculosis at this institute. Less favorable test characteristics were obtained for patients with miliary and pleural tuberculosis, in which the test had sensitivities of 22% and 14%. These results offer the promise of an accurate serodiagnostic test for pulmonary tuberculosis using readily obtained reagents.
在墨西哥城的国家呼吸疾病研究所建立了一种酶联免疫吸附测定法（ELISA），用于检测人血清中针对结核分枝杆菌30000道尔顿天然抗原的IgG抗体，并评估其在结核病诊断中的效用。该检测方法在痰涂片阳性的活动性肺结核患者中的敏感性为70%，在对照受试者中的特异性为100%。在该研究所，肺结核患者的阳性预测准确率为100%，阴性预测准确率为93%。粟粒性肺结核和胸膜炎性肺结核患者的检测结果较差，检测敏感性分别为22%和14%。这些结果表明，使用易于获得的试剂有望实现一种准确的肺结核血清学诊断检测方法。
