Department of Geriatrics, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, PR China.
Clin Biochem. 2011 Apr;44(5-6):386-90. doi: 10.1016/j.clinbiochem.2010.12.013. Epub 2010 Dec 31.
To investigate the association of coronary artery disease (CAD) and ischemic heart failure (IHF) with polymorphisms of the ghrelin gene in elderly Chinese patients.
Fifty-six patients with ischemic heart failure, sixty patients with coronary artery disease without heart failure, and one hundred healthy control subjects participated in the study. The polymorphisms were evaluated by polymerase chain reaction, sequencing, and fragment length polymorphism analysis.
Only one single nucleotide polymorphism (SNP), Leu72Met (408C/A), was observed across all samples. Gene frequencies of CC and allele frequencies of C were significantly greater in the CAD with IHF group than those in the CAD without IHF group (p=0.025, p=0.011). There was no significant association between the Leu72Met SNP with coronary artery disease risk factors.
Our results suggest that a C allele at position 408 of the ghrelin gene is associated with genetic susceptibility to ischemic heart failure in Chinese elders.
探讨中国老年患者中 ghrelin 基因多态性与冠状动脉疾病（CAD）和缺血性心力衰竭（IHF）的关系。
56 例缺血性心力衰竭患者、60 例无心力衰竭的 CAD 患者和 100 例健康对照者参与了这项研究。通过聚合酶链反应、测序和片段长度多态性分析来评估多态性。
所有样本中仅观察到一个单核苷酸多态性（SNP），即 Leu72Met（408C/A）。在 CAD 合并 IHF 组中，CC 基因频率和 C 等位基因频率明显高于 CAD 无 IHF 组（p=0.025，p=0.011）。Leu72Met SNP 与冠状动脉疾病危险因素之间无显著相关性。
我们的研究结果表明，ghrelin 基因第 408 位的 C 等位基因与中国老年人缺血性心力衰竭的遗传易感性相关。
