Li Chang-yu, Wang Xiao-lin, Wang Jian-hua, Yan Zhi-ping, Gong Gao-quan, Cheng Jie-min, Chen Yi, Liu Ling-xiao, Li Guo-ping, Wang Cheng-gang, Shi Dong-hua
Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai, China.
Front Biosci (Elite Ed). 2011 Jan 1;3(1):212-20. doi: 10.2741/e235.
Transcatheter Arterial Chemoembolization (TACE) is the first line of treatment in inoperable hepatocellular carcinoma. Magnetic affinity beads can be used to extract peptides from un-fractionated serum samples. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can detect the presence and the molecular mass of peptides. In this study, we used a highly optimized ClinProt-matrix-assisted laser desorption/ionization time-of flight mass spectrometer (MALDI-TOF-MS) to screen hepatocellular carcinoma markers for TACE. 40 sera from 20 patients, including before and after TACE to explore those biomarkers, might be related with therapy efficiency, and some of the patients who received another therapy were analyzed as well. The spectra were analyzed statistically using FlexAnalysis™ and Clin-Prot™ bioinformatic software. The seven most significant differential peaks (p < 0.0.5) were selected out by ClinProTool software to identify hepatocellular carcinoma markers for TACE therapy. Furthermore, the differential peptide of 3883Da was identified as plasma serine protease inhibitor precursor (Protein C inhibitor). This study provides a direct link between peptide marker profiles and TACE therapy, and the markers may have clinical utility for monitoring efficiency of therapy.
经导管动脉化疗栓塞术（TACE）是不可切除肝细胞癌的一线治疗方法。磁性亲和珠可用于从未经分离的血清样本中提取肽段。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）可检测肽段的存在及其分子量。在本研究中，我们使用高度优化的ClinProt-基质辅助激光解吸/电离飞行时间质谱仪（MALDI-TOF-MS）筛选用于TACE的肝细胞癌标志物。收集了20例患者的40份血清样本，包括TACE治疗前后的样本，以探索可能与治疗效果相关的生物标志物，同时也对部分接受其他治疗的患者进行了分析。使用FlexAnalysis™和Clin-Prot™生物信息软件对光谱进行统计学分析。通过ClinProTool软件选出七个最显著的差异峰（p<0.05），以鉴定用于TACE治疗的肝细胞癌标志物。此外，鉴定出分子量为3883Da的差异肽为血浆丝氨酸蛋白酶抑制剂前体（蛋白C抑制剂）。本研究建立了肽标志物谱与TACE治疗之间的直接联系，这些标志物可能在监测治疗效果方面具有临床应用价值。
