Liu Wentao, Gao Xiang, Cai Qu, Li Jianfang, Zhu Zhenglun, Li Chen, Yao Xuexin, Yang Qiumeng, Xiang Ming, Yan Min, Zhu Zhenggang
Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai Institute of Digestive Surgery, Shanghai 200025, PR China.
Front Biosci (Elite Ed). 2010 Jun 1;2(3):961-71. doi: 10.2741/e155.
Early diagnosis and early treatment is known to improve prognosis for gastric cancer. Magnetic affinity beads can be used to extract peptides from un-fractionated serum samples. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can detect the presence and the molecular mass of peptides. MALDI-TOF-MS mass spectra of peptides and proteins were generated after WCX CLINPROT bead fractionation of 62 gastric cancer serum samples. The discovery set consisted of 44 samples while the validation set was 18 serum samples. The spectra were analyzed statistically using flexAnalysisTM and Clin-ProtTM bioinformatic software. The six most significant peaks were selected out by ClinProTool software and utilized to train a Supervised Neural Network to identify gastric cancer sera from control sera. The sensitivity and specificity of the model when tested on the validation set were 100% and 75%, respectively. A set of 6 peptides that can be used to distinguish serum from gastric cancer patients with good sensitivity and specificity were identified, and these peptides may be useful biomarkers to distinguish cancer individuals who may benefit from radiologic or endoscopic examination.
众所周知，早期诊断和早期治疗可改善胃癌的预后。磁亲和珠可用于从未经分级分离的血清样本中提取肽段。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）可检测肽段的存在及其分子量。对62份胃癌血清样本进行WCX CLINPROT磁珠分级分离后，生成了肽段和蛋白质的MALDI-TOF-MS质谱图。发现集包括44个样本，验证集为18份血清样本。使用flexAnalysisTM和Clin-ProtTM生物信息软件对光谱进行统计分析。通过ClinProTool软件选出六个最显著的峰，并用于训练监督神经网络，以从对照血清中识别胃癌血清。该模型在验证集上测试时的灵敏度和特异性分别为100%和75%。鉴定出一组6种肽段，它们可用于以良好的灵敏度和特异性区分胃癌患者的血清，这些肽段可能是有助于区分可能从放射学或内镜检查中受益的癌症个体的生物标志物。
