Wang Hongxia, Zhang Fengchun, Ye Fei, Ma Yuan, Zhang David Y
Shanghai Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Front Biosci (Elite Ed). 2011 Jan 1;3(1):326-40. doi: 10.2741/e248.
The effects of Coptis chinensis on the behavior of squamous cell carcinoma have not yet been established. We examined the anticancer activity of Coptis chinensis on human squamous carcinoma cells, both in vitro and in xenografted nude mice, and applied Pathway Array Technology to understand possible involvement of signaling pathways in Coptis Chinensis induced tumor cells inhibition as well. Following Coptis chinensis treatment, a time-dependent reduction in proliferation was observed in both cell lines and NCR/NU mice. Coptis chinensis has a wide effect on cell signaling, including cell cycle regulation (Cdk6, Cdk4, cyclin B1, cyclin E, cyclin D1, p27), cell adhesion (E-cadherin, osteopontin), differentiation, apoptosis(p-Stat3, p53, BRCA1), cytoskeleton (p-PKC α/β II, Vimentin, p-PKCα), MAPK signaling (raf-1, ERK1/2, p-p38, p-ERK), and the phosphatidylinositol 3-kinase signaling pathway (p-Akt, Akt, p-PTEN). In our conclusions, Coptis chinensis may be a novel therapeutic drug for squamous cell carcinoma.
黄连对鳞状细胞癌行为的影响尚未明确。我们在体外和异种移植裸鼠中研究了黄连对人鳞状癌细胞的抗癌活性，并应用通路阵列技术来了解信号通路在黄连诱导肿瘤细胞抑制中可能的参与情况。黄连处理后，在两种细胞系和NCR/NU小鼠中均观察到增殖随时间的减少。黄连对细胞信号传导有广泛影响，包括细胞周期调控（Cdk6、Cdk4、细胞周期蛋白B1、细胞周期蛋白E、细胞周期蛋白D1、p27）、细胞黏附（E-钙黏蛋白、骨桥蛋白）、分化、凋亡（p-Stat3、p53、BRCA1）、细胞骨架（p-PKCα/β II、波形蛋白、p-PKCα）、MAPK信号传导（raf-1、ERK1/2、p-p38、p-ERK）以及磷脂酰肌醇3-激酶信号通路（p-Akt、Akt、p-PTEN）。我们的结论是，黄连可能是一种治疗鳞状细胞癌的新型药物。
