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黄连通过降低组蛋白去乙酰化酶3（HDAC3）来下调信号转导和转录激活因子3（STAT3）的磷酸化水平，从而影响胶质瘤细胞的功能。

Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3.

作者信息

Li Jiangan, Ni Lulu, Li Bing, Wang Mingdeng, Ding Zhemin, Xiong Chunrong, Lu Xiaojie

机构信息

Department of Neurosurgery, Wuxi No.2 Hospital Affiliated to Nanjing Medical University, No. 68 Zhongshan Road, Wuxi, 214000, People's Republic of China.

Department of Traditional Chinese medicin, the Affiliated Hospital of Jiangnan University ( Wuxi No.4 People's Hospital), Wuxi, 214062, People's Republic of China.

出版信息

BMC Complement Altern Med. 2017 Dec 6;17(1):524. doi: 10.1186/s12906-017-2029-0.

DOI:10.1186/s12906-017-2029-0
PMID:29212474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5719523/
Abstract

BACKGROUND

Glioma remains the most common cause of brain cancer-related mortality. Glioma accounts for 50-60% of brain cancer. Due to their low toxicity and infrequent side effects, traditional herbs have been increasingly popular. Coptis Chinensis is commonly used in cancer treatment in combination with other Chinese Medicine herbs. However, little is known about its biological functions and mechanisms in glioma cells.

METHODS

In this study, the anti-glioma cell effect of Coptis Chinensis was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method, plate clone test, scratch tests, flow cytometry, western blotting and a glioma xenograft tumor model.

RESULTS

The results showed that Coptis Chinensis significantly suppressed glioma cell proliferation, tumor formation, migration and tumor growth, and prolonged the survival time of glioma cell-bearing mice. The flow cytometry result showed that Coptis Chinensis induced cell cycle arrest and apoptosis in glioma cells. Western blotting showed that Coptis Chinensis down-regulated the Signal transducer and activator of transcription 3 (STAT3) phosphorylation levels and reduced the expression of Histone deacetylase 3 (HDAC3) and caspase 3.

CONCLUSIONS

Coptis Chinensis can inhibit various aspects of glioma cell functions. This study provides favorable scientific evidence for the potential use of natural products such as Coptis Chinensis in the clinical treatment of patients with glioma.

摘要

背景

胶质瘤仍然是脑癌相关死亡的最常见原因。胶质瘤占脑癌的50 - 60%。由于传统草药毒性低且副作用少，其越来越受欢迎。黄连常用于与其他中药联合治疗癌症。然而，其在胶质瘤细胞中的生物学功能和机制尚不清楚。

方法

在本研究中，采用3 -（4,5 - 二甲基 - 2 - 噻唑基）- 2,5 - 二苯基 - 2 - H - 四氮唑溴盐（MTT）法、平板克隆试验、划痕试验、流式细胞术、蛋白质印迹法和胶质瘤异种移植肿瘤模型来确定黄连对胶质瘤细胞的作用。

结果

结果表明，黄连显著抑制胶质瘤细胞增殖、肿瘤形成、迁移和肿瘤生长，并延长荷胶质瘤细胞小鼠的存活时间。流式细胞术结果显示，黄连诱导胶质瘤细胞周期停滞和凋亡。蛋白质印迹法显示，黄连下调信号转导和转录激活因子3（STAT3）的磷酸化水平，并降低组蛋白去乙酰化酶3（HDAC3）和半胱天冬酶3的表达。

结论

黄连可抑制胶质瘤细胞功能的各个方面。本研究为黄连等天然产物在胶质瘤患者临床治疗中的潜在应用提供了有力的科学证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/d3619c0d850f/12906_2017_2029_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/87a8d5b7ef33/12906_2017_2029_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/5fbb7df5b514/12906_2017_2029_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/5b95cb9995d7/12906_2017_2029_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/4fa3c874ff54/12906_2017_2029_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/c8b7b777f642/12906_2017_2029_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/e57dbfc49cdc/12906_2017_2029_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/68823c5b4eb9/12906_2017_2029_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/d3619c0d850f/12906_2017_2029_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/87a8d5b7ef33/12906_2017_2029_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/5fbb7df5b514/12906_2017_2029_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/5b95cb9995d7/12906_2017_2029_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/4fa3c874ff54/12906_2017_2029_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/c8b7b777f642/12906_2017_2029_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/e57dbfc49cdc/12906_2017_2029_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/68823c5b4eb9/12906_2017_2029_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb54/5719523/d3619c0d850f/12906_2017_2029_Fig8_HTML.jpg

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