Department of Oral Science, Federal University of Goiás, Rua C-245, Quadra 546, Lote 9, Jardim América, Goiânia, GO, CEP: 74.290-200, Brazil.
Med Oral Patol Oral Cir Bucal. 2011 May 1;16(3):e418-24. doi: 10.4317/medoral.16.e418.
The aim of this study was to compare the microbial leakage of mineral trioxide aggregate (MTA), Portland cement (PC), Sealapex and zinc oxide-eugenol (ZOE) as root-end filling materials.
An in vitro microbial leakage test (MLT) with a split chamber was used in this study. A mixture of facultative bacteria and one yeast (S. aureus+E. faecalis+P. aeruginosa+B. subtilis+C. albicans) was placed in the upper chamber and it could only reach the lower chamber containing Brain Heart Infusion broth by way of leakage through the root-end filling. Microbial leakage was observed daily for 60 days. Sixty maxillary anterior human teeth were randomly assigned to different groups--MTA and PC (gray and white), Sealapex+zinc oxide and ZOE, control groups and subgroups to evaluate the influence of EDTA for smear layer removal. These materials were further evaluated by an agar diffusion test (ADT) to verify their antimicrobial efficacy. Data were analyzed statistically by Kruskal-Wallis and Mann-Whitney test.
In the MLT, Sealapex+zinc oxide and ZOE did not show evidence of microbial leakage over the 60-day experimental period. The other materials showed leakage from the 15th day. The presence of smear layer influenced microbial leakage. Microbial inhibition zones were not observed in all samples tested by ADT.
Sealapex+zinc oxide and ZOE did not show microbial leakage over the experimental period, whereas it was verified within 15 to 45 days in MTA and Portland cement.
本研究旨在比较三氧化矿物聚合体（MTA）、波特兰水泥（PC）、Sealapex 和氧化锌丁香酚（ZOE）作为根管封闭材料的微生物渗漏情况。
本研究采用了一种带有分体室的体外微生物渗漏测试（MLT）。在这个实验中，需将兼性细菌和一种酵母（金黄色葡萄球菌+粪肠球菌+铜绿假单胞菌+枯草芽孢杆菌+白色念珠菌）的混合物放在上层腔室中，并且只有通过渗漏，才能让混合物到达下层腔室中含有脑心浸液肉汤的部分。微生物渗漏情况每天观察 60 天。60 颗上颌前牙被随机分配到不同的组中，即 MTA 和 PC（灰色和白色）、Sealapex+氧化锌和 ZOE、对照组和亚组，以评估 EDTA 对清除玷污层的影响。这些材料进一步通过琼脂扩散试验（ADT）进行评估，以验证其抗菌功效。数据通过 Kruskal-Wallis 和 Mann-Whitney 检验进行统计分析。
在 MLT 中，Sealapex+氧化锌和 ZOE 在 60 天的实验期间没有表现出微生物渗漏的证据。而其他材料在第 15 天就出现了渗漏。玷污层的存在影响了微生物的渗漏。在所有经过 ADT 测试的样本中，均未观察到微生物抑制带。
在实验期间，Sealapex+氧化锌和 ZOE 没有表现出微生物渗漏，但在 MTA 和波特兰水泥中，在 15 至 45 天内被证实有渗漏。
