Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Insect Mol Biol. 2011 Jun;20(3):311-21. doi: 10.1111/j.1365-2583.2010.01066.x. Epub 2011 Jan 4.
We investigated the effects of Wolbachia infection on mtDNA variation in spider mites by sequencing a portion of the mitochondrial cytochrome oxidase I (COI) gene from 198 individuals of known infection status. Four Wolbachia strains were described in the current study, namely wUrtOri1, wUrtOri2, wUrtOri3 and wUrtCon1. As predicted, the haplotype and nucleotide diversity were lower in infected individuals than in uninfected individuals. However, these mtDNA haplotype data are not entirely concordant with the surface protein of wolbachia (wsp) sequence data and both infected and uninfected individuals were found of the same haplotype. Although values of Tajima's D and Fu & Li's F were consistently less than zero for most infected groups, McDonald-Kreitman tests suggested that the patterns of variation were different from those expected under neutrality in only the uninfected group. Thus, the neutrality tests do not show a clear effect of Wolbachia infection on patterns of mtDNA variation and substitution in spider mites.
我们通过对 198 个已知感染状况的个体的线粒体细胞色素氧化酶 I（COI）基因的一部分进行测序，研究了沃尔巴克氏体感染对蜘蛛螨 mtDNA 变异的影响。本研究共描述了 4 种沃尔巴克氏体菌株，分别为 wUrtOri1、wUrtOri2、wUrtOri3 和 wUrtCon1。正如预测的那样，感染个体的单倍型和核苷酸多样性低于未感染个体。然而，这些 mtDNA 单倍型数据与沃尔巴克氏体的表面蛋白（wsp）序列数据不完全一致，并且感染和未感染的个体都具有相同的单倍型。尽管大多数感染群体的 Tajima's D 和 Fu & Li 的 F 值始终小于零，但 McDonald-Kreitman 检验表明，仅在未感染群体中，变异模式与中性预期的模式不同。因此，中性检验并未显示沃尔巴克氏体感染对蜘蛛螨 mtDNA 变异和替代模式的明显影响。
