Chen L B, Chen P L, Wang G L, Song X T, Li Y F, Liu C Y, Jia K M
PLA Institute of Hepatology, Beijing.
Chin Med J (Engl). 1990 Jun;103(6):447-50.
Liver specimens from the Shanghai Ma ducks congenitally infected with duck hepatitis B virus (DHBV) were examined by immunohistochemical technique and electron microscopy. All the 12 serum DHBV positive ducks showed varying degrees of positive straining in hepatocytes. In 8 of the 12 ducks, DHBV antigen was discovered in the cytoplasm of biliary epithelial cells. Conventional electron microscopy revealed two kinds of virus particles in the biliary epithelial cells: 1. the incomplete virus, 40-50 nm in diameter and spherical in shape with an outer membrane, located mainly in the dilated cisternae of rough endoplasmic reticulum of the cells in large amounts; 2. the complete virions, 55-60 nm in diameter, were spherical with an outer membrane and located mainly in the cytoplasmic vesicles in small amount. We believe the particles found in the biliary epithelial cells in this study were DHBV particles. It is most likely that the infection and replication of DHBV not only take place in the liver cells but also in the biliary epithelial cells.
采用免疫组化技术和电子显微镜对先天性感染鸭乙型肝炎病毒（DHBV）的上海麻鸭肝脏标本进行检查。12只血清DHBV阳性鸭的肝细胞均呈现不同程度的阳性染色。12只鸭中有8只在胆管上皮细胞的细胞质中发现了DHBV抗原。常规电子显微镜在胆管上皮细胞中发现了两种病毒颗粒：1. 不完全病毒，直径40 - 50nm，球形，有外膜，大量存在于细胞粗面内质网扩张的潴泡中；2. 完整病毒粒子，直径55 - 60nm，球形，有外膜，少量存在于细胞质囊泡中。我们认为本研究在胆管上皮细胞中发现的颗粒为DHBV颗粒。很可能DHBV的感染和复制不仅发生在肝细胞中，也发生在胆管上皮细胞中。
