Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK.
Vaccine. 2011 Feb 17;29(9):1836-43. doi: 10.1016/j.vaccine.2010.12.044. Epub 2011 Jan 1.
Wild type human influenza viruses do not usually grow well in embryonated hens' eggs, the substrate of choice for the production of inactivated influenza vaccine, and vaccine viruses need to be developed specifically for this purpose. In the event of a pandemic of influenza, vaccine viruses need to be created with utmost speed. At the onset of the current A(H1N1) pandemic in April 2009, a network of laboratories began a race against time to develop suitable candidate vaccine viruses. Two approaches were followed, the classical reassortment approach and the more recent reverse genetics approach. This report describes the development and the characteristics of current pandemic H1N1 candidate vaccine viruses.
野生型人流感病毒通常在鸡胚中不能很好地生长，而鸡胚是生产灭活流感疫苗的首选基质，因此需要专门开发疫苗病毒。在流感大流行的情况下，需要以最快的速度创建疫苗病毒。在 2009 年 4 月当前的 A(H1N1)大流行开始时，一个实验室网络开始了一场与时间的赛跑，以开发合适的候选疫苗病毒。采用了两种方法，经典的重配方法和最近的反向遗传学方法。本报告描述了当前大流行 H1N1 候选疫苗病毒的开发和特性。
