使用经丝裂霉素C处理的培养物滤液对三种检测大肠杆菌细胞毒素坏死因子（CNF1和CNF2）的试验进行比较评估。
Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C.
作者信息
Blanco J, Blanco M, González E A, Alonso M P, Garabal J I
机构信息
Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago, Lugo, Spain.
出版信息
FEMS Microbiol Lett. 1990 Jun 1;57(3):311-6. doi: 10.1016/0378-1097(90)90086-6.
Necrotizing Escherichia coli (NTEC) strains grown in the presence of mitomycin C released cell associated necrotizing factors CNF1 and CNF2 to culture medium. Using culture filtrates from 96 mitomycin C treated E. coli strains, we have found that a modified HeLa cell assay was a more sensitive and specific method for the detection of CNF1 and CNF2 than the Vero cell assay and the rabbit skin test.
在丝裂霉素C存在的情况下生长的坏死性大肠杆菌（NTEC）菌株将细胞相关坏死因子CNF1和CNF2释放到培养基中。使用来自96株经丝裂霉素C处理的大肠杆菌菌株的培养滤液，我们发现改良的HeLa细胞检测法比Vero细胞检测法和兔皮试验在检测CNF1和CNF2方面是一种更灵敏且特异的方法。
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