Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.
Antonie Van Leeuwenhoek. 2011 Mar;99(3):711-20. doi: 10.1007/s10482-010-9546-x. Epub 2011 Jan 4.
Three different bacterial species are regularly described from tsetse flies. However, no broad screens have been performed to investigate the existence of other bacteria in this medically and agriculturally important vector insect. Utilising both culture dependent and independent methods we show that Kenyan populations of Glossina fuscipes fuscipes harbour a surprising diversity of bacteria. Bacteria were isolated from 72% of flies with 23 different bacterial species identified. The Firmicutes phylum dominated with 16 species of which seven belong to the genus Bacillus. The tsetse fly primary symbiont, Wigglesworthia glossinidia, was identified by the culture independent pathway. However, neither the secondary symbiont Sodalis nor Wolbachia was detected with either of the methods used. Two other bacterial species were identified with the DNA based method, Bacillus subtilis and Serratia marcescens. Further studies are needed to determine how tsetse flies, which only ever feed on vertebrate blood, pick up bacteria and to investigate the possible impact of these bacteria on Glossina longevity and vector competence.
三种不同的细菌物种经常从采采蝇中描述。然而，没有广泛的筛选来调查这种在医学和农业上重要的媒介昆虫中其他细菌的存在。利用依赖培养和独立的方法，我们表明肯尼亚的褐须角蝇种群栖息着令人惊讶的细菌多样性。从 72%的苍蝇中分离出细菌，鉴定出 23 种不同的细菌物种。厚壁菌门占主导地位，其中有 16 种属于芽孢杆菌属。采采蝇的主要共生菌，Wigglesworthia glossinidia，通过非培养途径鉴定。然而，无论是哪种方法都没有检测到次要共生菌 Sodalis 和 Wolbachia。另两种细菌种是通过基于 DNA 的方法鉴定的，枯草芽孢杆菌和粘质沙雷氏菌。需要进一步研究来确定只吸食脊椎动物血液的采采蝇如何获得细菌，并调查这些细菌对采采蝇寿命和媒介能力的可能影响。
