共生细菌格氏嗜 Sodalis glossinidius、螺旋体属（Spiroplasma sp）和沃尔巴克氏体（Wolbachia）不利于布基纳法索博博迪乌拉索采集的采采蝇野生种群中灰色锥虫（Trypanosoma grayi）的共存。

Symbiotic bacteria Sodalis glossinidius, Spiroplasma sp and Wolbachia do not favour Trypanosoma grayi coexistence in wild population of tsetse flies collected in Bobo-Dioulasso, Burkina Faso.

作者信息

Mfopit Youssouf Mouliom, Bilgo Etienne, Boma Soudah, Somda Martin Bienvenu, Gnambani Jacques Edounou, Konkobo Maurice, Diabate Abdoulaye, Dayo Guiguigbaza-Kossigan, Mamman Mohammed, Kelm Soerge, Balogun Emmanuel Oluwadare, Shuaibu Mohammed Nasir, Kabir Junaidu

机构信息

Institute of Agricultural Research for Development (IRAD).

Institut de Recherche en Sciences de la Santé.

出版信息

Res Sq. 2024 Aug 28:rs.3.rs-4756528. doi: 10.21203/rs.3.rs-4756528/v1.

BACKGROUND

Tsetse flies, the biological vectors of African trypanosomes, have established symbiotic associations with different bacteria. Their vector competence is suggested to be affected by bacterial endosymbionts. The current study provided the prevalence of three tsetse symbiotic bacteria and trypanosomes in species from Burkina Faso.

RESULTS

A total of 430 tsetse flies were captured using biconical traps in four different collection sites around Bobo-Dioulasso (Bama, Bana, Nasso, and Peni), and their guts were removed. Two hundred tsetse were randomly selected and their guts were screened byPCR for the presence of ., and trypanosomes. Of the 200 tsetse, 196 (98.0%) were and 4 (2.0%) . The overall symbiont prevalence was 49.0%, 96.5%, and 45.0%, respectively for and . Prevalence varied between sampling locations: (54.7%, 38.5%, 31.6%, 70.8%); (100%, 100%, 87.7%, 100%); and (43.4%, 38.5%, 38.6%, 70.8%),respectively in Bama, Bana, Nasso and Peni. Noteworthy, no was infected by . and , but they were all infected by . A total of 196 (98.0 %) harbored at least one endosymbionts. Fifty-five (27.5%) carried single endosymbiont. Trypanosomes were found only in , but not . Trypanosomes were present in flies from all study locations with an overall prevalence of 29.5%. In Bama, Bana, Nasso, and Peni, the trypanosome infection rate was respectively 39.6%, 23.1%, 8.8%, and 37.5%. Remarkably, only was present. Of all trypanosome-infected flies, 55.9%, 98.3%, and 33.9% hosted and , respectively. There was no association between , and trypanosome presence, but there was a negative association with presence. We reported1.9 times likelihood of trypanosome absence when was present.

CONCLUSION

This is the first survey reporting the presence of in tsetse from Burkina Faso. Tsetse from these localities were highly positive for symbiotic bacteria, more predominantly with . Modifications of symbiotic interactions may pave way for disease control.

背景

采采蝇是非洲锥虫的生物传播媒介，已与不同细菌建立了共生关系。其传播能力被认为受细菌内共生体影响。本研究提供了布基纳法索三种采采蝇共生细菌和锥虫的流行情况。

结果

在博博迪乌拉索周围四个不同采集点（巴马、巴纳、纳索和佩尼）使用双锥陷阱共捕获430只采采蝇，并取出其肠道。随机选择200只采采蝇，通过聚合酶链反应（PCR）检测其肠道中是否存在[细菌名称1]、[细菌名称2]和锥虫。在这200只采采蝇中，196只（98.0%）为[采采蝇种类1]，4只（2.0%）为[采采蝇种类2]。[细菌名称1]、[细菌名称2]和[细菌名称3]的总体共生体流行率分别为49.0%、96.5%和45.0%。不同采样地点的流行率有所不同：在巴马、巴纳、纳索和佩尼，[细菌名称1]的流行率分别为54.7%、38.5%、31.6%、70.8%；[细菌名称2]的流行率分别为100%、100%、87.7%、100%；[细菌名称3]的流行率分别为43.4%、38.5%、38.6%、70.8%。值得注意的是，没有[采采蝇种类2]被[细菌名称1]、[细菌名称2]和[细菌名称3]感染，但它们都被[另一种细菌名称]感染。共有196只（98.0%）采采蝇至少携带一种内共生体。55只（27.5%）携带单一内共生体。仅在[采采蝇种类1]中发现锥虫，在[采采蝇种类2]中未发现。所有研究地点的采采蝇中均存在锥虫，总体流行率为29.5%。在巴马、巴纳、纳索和佩尼，锥虫感染率分别为39.6%、23.1%、8.8%和37.5%。值得注意的是，仅发现[某种锥虫种类]。在所有感染锥虫的采采蝇中，分别有55.9%、98.3%和33.9%携带[细菌名称1]、[细菌名称2]和[细菌名称3]。[细菌名称1]、[细菌名称2]与锥虫的存在之间没有关联，但与[另一种细菌名称]的存在呈负相关。我们报告当存在[另一种细菌名称]时，锥虫不存在的可能性为1.9倍。