Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK.
Biochem J. 2011 Mar 1;434(2):211-7. doi: 10.1042/BJ20101772.
A male infertility-linked human PLCζ (phospholipase Cζ) mutation introduced into mouse PLCζ completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca2+ oscillations in mouse eggs. Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism.
一个与男性不育相关的人 PLCζ（磷脂酶 Cζ）突变被引入小鼠 PLCζ 中，完全消除了体外 PIP2（磷脂酰肌醇 4,5-二磷酸）水解活性和在小鼠卵子中引发体内 Ca2+ 振荡的能力。在存在 10 倍更高突变型 PLCζ 的情况下，野生型 PLCζ 在卵子中引发了正常的 Ca2+ 振荡模式，表明不育不是由显性负性机制介导的。
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