Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská, České Budějovice, Czech Republic.
Parasite Immunol. 2011 Apr;33(4):210-6. doi: 10.1111/j.1365-3024.2010.01271.x.
The role of CD4+ and CD8+ T lymphocytes in the development of a protective immune response against Cryptosporidium muris infection was studied by the reconstitution of severe combined immunodeficient (SCID) mice with well-defined populations of either naive or immune CD8+ or CD4+ T lymphocytes. Adoptive transfer of both naive and immune CD4+ T lymphocyte subpopulations protects SCID mice against cryptosporidiosis. Moreover, a significant biological impact of activated CD8+ T cells against gastric cryptosporidiosis was observed. The significant difference in the course and intensity of the infection in reconstituted SCID mice was found to be dependent on the protective function of both the CD4+ and CD8+ T-cell populations transferred. While SCID mice reconstituted with either immune or naive CD4+ or immune CD8+ T-cell subpopulations resolved the infection within 29, 37 and 51 days post-infection, respectively, those reconstituted with naive CD8+ T cells suffered from chronic infection similar to control SCID mice. Reconstitution with CD4+ T cells resulted in suppression of oocyst excretion and shortening of patent period in comparison with SCID mice reconstituted with CD8+ T cells. Thus, although CD4+ T cells are considered important in protective immunity, our results are the first to demonstrate the involvement of activated CD8+ T lymphocytes in the protection of mice against gastric cryptosporidiosis.
研究了 CD4+和 CD8+T 淋巴细胞在抗鼠隐孢子虫感染的保护性免疫反应发展中的作用，方法是用定义明确的幼稚或免疫 CD8+或 CD4+T 淋巴细胞群体重建严重联合免疫缺陷（SCID）小鼠。幼稚和免疫 CD4+T 淋巴细胞亚群的过继转移均可保护 SCID 小鼠免受隐孢子虫病的侵害。此外，还观察到激活的 CD8+T 细胞对胃隐孢子虫病具有显著的生物学影响。发现重建的 SCID 小鼠感染过程和强度的显著差异取决于转移的 CD4+和 CD8+T 细胞群体的保护功能。尽管用幼稚或免疫 CD4+或免疫 CD8+T 细胞亚群重建的 SCID 小鼠在感染后 29、37 和 51 天分别消除了感染，但用幼稚 CD8+T 细胞重建的 SCID 小鼠则遭受类似于对照 SCID 小鼠的慢性感染。与用 CD8+T 细胞重建的 SCID 小鼠相比，用 CD4+T 细胞重建导致卵囊排泄减少和发病期缩短。因此，尽管 CD4+T 细胞被认为在保护性免疫中很重要，但我们的结果是首次证明激活的 CD8+T 淋巴细胞参与了对小鼠胃隐孢子虫病的保护。
