McDonald V, Robinson H A, Kelly J P, Bancroft G J
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, United Kingdom.
Infect Immun. 1994 Jun;62(6):2289-94. doi: 10.1128/iai.62.6.2289-2294.1994.
The aim of this study was to investigate the role of the CD4 and CD8 T cells in immunity to cryptosporidia by using Cryptosporidium muris and a mouse model of infection. Two approaches were used, each involving the use of rat anti-T-cell surface marker monoclonal antibodies (MAbs). In the first, the adoptive transfer of immunity was studied by using the CB.17 SCID mouse (which lacks T and B cells) as the host; in the second, the effect on susceptibility of BALB/c mice to infection was examined following depletion of T cells or subsets of T cells. In adoptive immunity experiments, the conditions which differentiated between resistance associated with reconstitution of SCID mice with naive BALB/c lymphocytes and the transfer of immunity with primed lymphocytes from infected animals were determined. Primed spleen or mesenteric lymph node cells conferred better protection to recipients than naive cells when obtained from donors which had developed resistance to infection. Adoptive immunity was abrogated when Thy.1 cells or CD4 cells were depleted from primed cells, while depletion of CD8 cells could reduce the level of protection. In the study of C. muris in BALB/c mice, treatment with either anti-Thy.1 plus anti-Lyt.1 or anti-CD4 MAbs increased susceptibility to a primary infection as determined by the size and duration of oocyst production, but an anti-CD8 MAb produced an increase only in oocyst shedding. Thus, both CD4 and, to a lesser extent, CD8 cells appeared to be involved in resistance to primary and secondary C. muris infection.
本研究的目的是通过使用微小隐孢子虫和小鼠感染模型来研究CD4和CD8 T细胞在隐孢子虫免疫中的作用。采用了两种方法，每种方法都涉及使用大鼠抗T细胞表面标志物单克隆抗体（MAb）。第一种方法是，以CB.17 SCID小鼠（缺乏T细胞和B细胞）作为宿主研究免疫的过继转移；第二种方法是，在T细胞或T细胞亚群耗竭后，检查其对BALB/c小鼠感染易感性的影响。在过继免疫实验中，确定了区分用未致敏的BALB/c淋巴细胞重建SCID小鼠相关的抵抗力和用感染动物的致敏淋巴细胞转移免疫的条件。当从已产生感染抵抗力的供体获得时，致敏的脾细胞或肠系膜淋巴结细胞比未致敏的细胞能为受体提供更好的保护。当从致敏细胞中去除Thy.1细胞或CD4细胞时，过继免疫被消除，而去除CD8细胞可降低保护水平。在对BALB/c小鼠微小隐孢子虫的研究中，用抗Thy.1加抗Lyt.1或抗CD4单克隆抗体治疗会增加对初次感染的易感性，这可通过卵囊产生的大小和持续时间来确定，但抗CD8单克隆抗体仅使卵囊排出增加。因此，CD4细胞以及程度较轻的CD8细胞似乎都参与了对微小隐孢子虫初次和二次感染的抵抗力。
