Department of Virology, Parasitology and Immunology, Ghent University. Salisburylaan 133, Merelbeke, Belgium.
Parasite Immunol. 2011 Apr;33(4):250-4. doi: 10.1111/j.1365-3024.2010.01274.x.
To determine whether purified Ascaris suum haemoglobin (AsHb) is a suitable vaccine candidate for the control of Ascaris infections, pigs were vaccinated with AsHb in combination with QuilA adjuvant and challenged with A. suum eggs. The number of liver lesions and worms in the intestine was assessed on day 14, 28 and 56 post-infection (p.i.). No significant differences were found in the number of worms recovered between vaccinated and control pigs on any of these days. However, significantly more white spots were counted on the livers of vaccinated pigs on day 14 (+86%) and day 28 (+118%) p.i. compared with nonvaccinated controls. To investigate whether the increased immunoreactivity against the liver stage L3s in vaccinated pigs was triggered by and directed against AsHb, the transcription and expression of AsHb in this larval life stage was analysed by RT-PCR and immunoblotting. The results showed that neither the AsHb transcript nor protein was detectable in freshly hatched L3. However, the immunoblot analysis showed that vaccination with AsHb resulted in the production of antibodies binding to several other antigens of the L3, suggesting that these might be involved in the increased white spot development.
为了确定纯化的猪蛔虫血红蛋白（AsHb）是否是一种合适的疫苗候选物，用于控制蛔虫感染，我们用 AsHb 联合 QuilA 佐剂对猪进行了疫苗接种，并进行了猪蛔虫卵攻毒实验。在攻毒后第 14、28 和 56 天，评估了肝脏病变和肠道内的蛔虫数量。在这些天的任何一天，接种组和对照组猪体内回收的蛔虫数量均无显著差异。然而，与未接种对照组相比，接种组猪的肝脏上的白色斑点数量在攻毒后第 14 天（+86%）和第 28 天（+118%）时显著增加。为了研究接种猪肝脏期 L3s 中增强的免疫反应是否由 AsHb 触发并针对其产生，我们通过 RT-PCR 和免疫印迹分析了该幼虫生活阶段中 AsHb 的转录和表达。结果表明，刚孵化的 L3 中既没有检测到 AsHb 转录本，也没有检测到 AsHb 蛋白。然而，免疫印迹分析表明，用 AsHb 进行疫苗接种会导致产生针对 L3 的几种其他抗原的抗体，这表明这些抗原可能参与了白色斑点的形成。
