Welsh School of Pharmacy, Cardiff University, Cardiff, Wales, UK.
J Appl Microbiol. 2011 Mar;110(3):631-40. doi: 10.1111/j.1365-2672.2010.04928.x. Epub 2011 Jan 12.
Pseudomonas aeruginosa is an opportunistic pathogen and is the main cause of respiratory infection in cystic fibrosis patients. Most strains prevalent within the UK are resistant to two or more antibiotics leading to the search for new therapeutic strategies including the use of bacteriophages.
The infectivity of four bacteriophages was increased using an enhancement protocol based on the use of pomegranate rind extract. Their efficacy against 14 Ps. aeruginosa strains was measured using a qualitative streak test and a novel quantitative assay based on the Bioscreen C microbial growth analyzer. Streak test analysis illustrated an increase in the lytic activity of enhanced bacteriophages, whereas Bioscreen analysis showed that both enhanced and unenhanced bacteriophages failed to meet acceptable levels of activity in c. 50% of strains tested.
The quantitative Bioscreen C analyzer showed comparable but not identical results in phage activity and identified significant bacterial re-growth by 20 h postinfection.
With the resurgence of interest in bacteriophage therapy against infectious bacterial diseases, a rapid high throughput quantitative method for screening phage activity and bacterial resistance is required. The use of the Bioscreen C analyzer meets these criteria and was shown to be more stringent than the traditional streak test.
铜绿假单胞菌是一种机会性病原体，是囊性纤维化患者呼吸道感染的主要原因。在英国流行的大多数菌株对两种或两种以上的抗生素具有耐药性，导致人们寻求新的治疗策略，包括使用噬菌体。
使用基于石榴皮提取物使用的增强方案来提高四种噬菌体的感染力。使用定性划线试验和基于 Bioscreen C 微生物生长分析仪的新型定量测定法来测量它们对 14 株铜绿假单胞菌菌株的功效。划线试验分析表明，增强噬菌体的裂解活性增加，而 Bioscreen 分析表明，增强和未增强的噬菌体在测试的约 50%的菌株中均未达到可接受的活性水平。
定量 Bioscreen C 分析仪在噬菌体活性方面显示出可比但不相同的结果，并在感染后 20 小时发现细菌明显重新生长。
随着人们对噬菌体治疗传染性细菌疾病的兴趣重新兴起，需要一种快速、高通量的筛选噬菌体活性和细菌耐药性的定量方法。Bioscreen C 分析仪的使用符合这些标准，并且比传统的划线试验更为严格。
