分离和鉴定噬菌体能源 Yersinia phage fMtkYen3-01。

Isolation and characterization of Yersinia phage fMtkYen3-01.

机构信息

Department of Biological and Environmental Science and Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland.

Human Microbiome Research Program, Department of Bacteriology and Immunology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.

出版信息

Arch Virol. 2024 Oct 19;169(11):226. doi: 10.1007/s00705-024-06149-6.

DOI:10.1007/s00705-024-06149-6

PMID:39425798

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11490452/

Abstract

Yersinia enterocolitica causes yersiniosis, the third most common gastrointestinal infection in humans throughout Europe. The emergence of multidrug resistance and the lack of effective new antibiotics have drawn attention to phage therapy as a treatment option. Here, we report the complete genome sequence of phage fMtkYen3-01, which infects Y. enterocolitica serotype O:3 strains. This phage has a genome 40,415 bp in length with 45.1% GC content and 49 predicted genes. fMtkYen3-01 infected 9.5% of the 42 Y. enterocolitica strains tested and showed stability at 25-40 °C, as well as pH 5.0-10.0. These results suggest the therapeutic potential of this phage.

摘要

肠炎沙门氏菌引起肠炎沙门氏菌病，是整个欧洲第三大常见的人类胃肠道感染。多药耐药性的出现和缺乏有效的新型抗生素引起了人们对噬菌体疗法作为一种治疗选择的关注。在这里，我们报告了感染肠炎沙门氏菌血清型 O:3 菌株的噬菌体 fMtkYen3-01 的完整基因组序列。该噬菌体基因组全长 40415 bp，GC 含量为 45.1%，预测有 49 个基因。fMtkYen3-01 感染了 42 株肠炎沙门氏菌中 9.5%的菌株，在 25-40°C 和 pH 5.0-10.0 条件下具有稳定性。这些结果表明该噬菌体具有治疗潜力。