Centre of Excellence for Biosensors, Instrumentation and Process Control, Ajdovščina, Slovenia.
Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Domžale, Slovenia.
Front Cell Infect Microbiol. 2023 May 24;13:1169135. doi: 10.3389/fcimb.2023.1169135. eCollection 2023.
is an important opportunistic pathogen causing chronic prosthetic joint infections associated with biofilm growth. Increased tolerance to antibiotic therapy often requires prolonged treatment or revision surgery. Phage therapy is currently used as compassionate use therapy and continues to be evaluated for its viability as adjunctive therapy to antibiotic treatment or as an alternative treatment for infections caused by to prevent relapses. In the present study, we report the isolation and characterization of three novel lytic phages. Their genome content analysis indicated the absence of antibiotic resistance genes and virulence factors. Detailed investigation of the phage preparation indicated the absence of any prophage-related contamination and demonstrated the importance of selecting appropriate hosts for phage development from the outset. The isolated phages infect a high proportion of clinically relevant strains and several other coagulase-negative species growing both in planktonic culture and as a biofilm. Clinical strains differing in their biofilm phenotype and antibiotic resistance profile were selected to further identify possible mechanisms behind increased tolerance to isolated phages.
是一种重要的机会性病原体，可导致慢性人工关节感染，并与生物膜生长有关。对抗生素治疗的耐受性增加通常需要延长治疗时间或进行翻修手术。噬菌体治疗目前被用作同情用药治疗，并继续被评估作为抗生素治疗的辅助治疗或作为预防复发的感染的替代治疗方法。在本研究中，我们报告了三种新型裂解噬菌体的分离和特性。它们的基因组内容分析表明不存在抗生素耐药基因和毒力因子。对噬菌体制剂的详细调查表明不存在任何与噬菌体相关的污染，并且从一开始就证明了选择合适的宿主用于噬菌体开发的重要性。分离的噬菌体感染了很大比例的临床相关菌株和其他几种凝固酶阴性种，无论是在浮游培养物中还是作为生物膜生长。选择了在生物膜表型和抗生素耐药谱上存在差异的临床菌株，以进一步确定对分离噬菌体的耐受性增加背后的可能机制。
