Leonardo F C, da Cunha A F, da Silva M J, Carazzolle M F, Costa-Leonardo A M, Costa F F, Pereira G A
Laboratório de Genômica e Expressão, Departamento de Genética Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, São Paulo, Brazil.
Bull Entomol Res. 2011 Aug;101(4):383-91. doi: 10.1017/S0007485310000556. Epub 2010 Dec 21.
The lower termite, Coptotermes gestroi (Isoptera: Rhinotermitidae), is originally from Southeast Asia and has become a pest in Brazil. The main goal of this study was to survey C. gestroi transcriptome composition. To accomplish this, we sequenced and analyzed 3003 expressed sequence tags (ESTs) isolated from libraries of worker heads. After assembly, 695 uniESTs were obtained from which 349 have similarity with known sequences. Comparison with insect genomes demonstrated similarity, primarily with genes from Apis mellifera (28%), Tribolium castaneum (28%) and Aedes aegypti (10%). Notably, we identified two endogenous cellulases in the sequences, which may be of interest for biotechnological applications. The results presented in this work represent the first genomic study of the Asian subterranean termite, Coptotermes gestroi.
低等白蚁——黑胸散白蚁（等翅目：鼻白蚁科）原产于东南亚，现已成为巴西的一种害虫。本研究的主要目的是调查黑胸散白蚁的转录组组成。为实现这一目标，我们对从工蚁头部文库中分离出的3003个表达序列标签（EST）进行了测序和分析。组装后，获得了695个单一EST，其中349个与已知序列相似。与昆虫基因组的比较显示出相似性，主要与意大利蜜蜂（28%）、赤拟谷盗（28%）和埃及伊蚊（10%）的基因相似。值得注意的是，我们在序列中鉴定出了两种内源性纤维素酶，这可能在生物技术应用中具有重要意义。本研究结果代表了对亚洲地下白蚁——黑胸散白蚁的首次基因组研究。
