广宿主范围质粒RP4整合到黄色粘球菌染色体中的机制。
Mechanism of integration of the broad-host-range plasmid RP4 into the chromosome of Myxococcus xanthus.
作者信息
Jaoua S, Letouvet-Pawlak B, Monnier C, Guespin-Michel J F
机构信息
Laboratoire de Microbiologie, CNRS URA 203, Faculté des Sciences et Techniques de Rouen, Mont Saint Aignan, France.
出版信息
Plasmid. 1990 May;23(3):183-93. doi: 10.1016/0147-619x(90)90050-m.
The site-specific recombination mechanism through which the plasmid RP4 has been previously shown to integrate into the chromosome of Myxococcus xanthus has been investigated further. Once integrated in one of the numerous chromosomal sites from two different strains, through a precise site on the plasmid, the latter can be excised either precisely or after a definite 14.5-kb deletion. In some cases, the integration is followed by different DNA rearrangements that yield a higher rate of excision and integration. A model for the site-specific integration and excision of the plasmid is proposed.
先前已证明质粒RP4通过位点特异性重组机制整合到黄色黏球菌染色体中，对此进行了进一步研究。一旦通过质粒上的精确位点整合到来自两个不同菌株的众多染色体位点之一中，后者可以精确切除，也可以在明确缺失14.5 kb后切除。在某些情况下，整合后会发生不同的DNA重排，从而产生更高的切除和整合率。提出了质粒位点特异性整合和切除的模型。
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