Glomp I, Saulnier P, Guespin-Michel J, Schairer H U
Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
Mol Gen Genet. 1988 Oct;214(2):213-7. doi: 10.1007/BF00337713.
Derivatives of the broad-host-range plasmid RP4, containing the wild-type or modified transposon Tn5 were transferred by conjugation to various Stigmatella aurantiaca isolates. The transposons and in some cases fragments of the plasmid as well were integrated into the chromosome. Thus, insertional mutants have been obtained affected in spore formation in liquid culture.
含有野生型或修饰型转座子Tn5的广宿主范围质粒RP4的衍生物,通过接合转移到各种橙黄粘球菌分离株中。转座子以及在某些情况下质粒片段也整合到了染色体中。因此,获得了在液体培养中孢子形成受到影响的插入突变体。