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转导至黄色粘球菌后Tn5介导的质粒DNA转座

Tn5-mediated transposition of plasmid DNA after transduction to Myxococcus xanthus.

作者信息

Downard J S

机构信息

Department of Botany and Microbiology, University of Oklahoma, Norman 73019.

出版信息

J Bacteriol. 1988 Oct;170(10):4939-41. doi: 10.1128/jb.170.10.4939-4941.1988.

DOI:10.1128/jb.170.10.4939-4941.1988
PMID:2844730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211541/
Abstract

After coliphage P1-mediated transfer of Tn5-containing plasmid DNA from Escherichia coli to Myxococcus xanthus, transductants were identified which contained plasmid sequences integrated at many sites on the bacterial chromosome. The unaltered plasmid DNA sequences in these transductants were apparently flanked by intact Tn5 or IS50 sequences. These results suggest that Tn5-mediated transposition has occurred and provide a method for integrating plasmid DNA into the M. xanthus chromosome without the requirement for homologous recombination.

摘要

在通过噬菌体P1介导将含Tn5的质粒DNA从大肠杆菌转移至黄色黏球菌后，鉴定出了转导子，其含有整合在细菌染色体多个位点上的质粒序列。这些转导子中未改变的质粒DNA序列显然侧翼为完整的Tn5或IS50序列。这些结果表明发生了Tn5介导的转座作用，并提供了一种将质粒DNA整合到黄色黏球菌染色体中而无需同源重组的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db97/211541/9da3ebf43fb8/jbacter00188-0522-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db97/211541/9da3ebf43fb8/jbacter00188-0522-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db97/211541/9da3ebf43fb8/jbacter00188-0522-a.jpg

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引用本文的文献

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Characterization of the temperate bacteriophage phi adh and plasmid transduction in Lactobacillus acidophilus ADH.嗜酸性乳杆菌ADH中温和噬菌体phi adh的特性及质粒转导

本文引用的文献

1
In situ transposon replacement and isolation of a spontaneous tandem genetic duplication.原位转座子替换及自发串联基因重复的分离
Mol Gen Genet. 1983;191(1):99-109. doi: 10.1007/BF00330896.
2
Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses.噬菌体温和噬菌体P1介导的克隆DNA从大肠杆菌到黄色粘球菌的转导：用于互补和重组分析。
J Bacteriol. 1983 Jul;155(1):317-29. doi: 10.1128/jb.155.1.317-329.1983.
3
Inverted repeats of Tn5 are transposable elements.
Appl Environ Microbiol. 1989 Sep;55(9):2206-13. doi: 10.1128/aem.55.9.2206-2213.1989.
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A segment of Myxococcus xanthus ops DNA functions as an upstream activation site for tps gene transcription.黄色黏球菌ops DNA的一个片段作为tps基因转录的上游激活位点发挥作用。
J Bacteriol. 1990 Jun;172(6):3081-8. doi: 10.1128/jb.172.6.3081-3088.1990.
Tn5的反向重复序列是可转座元件。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2632-5. doi: 10.1073/pnas.79.8.2632.
4
Gene expression during development of Myxococcus xanthus. Analysis of the genes for protein S.黄色粘球菌发育过程中的基因表达。蛋白质S相关基因的分析。
J Mol Biol. 1984 Jun 5;175(4):469-92. doi: 10.1016/0022-2836(84)90180-3.
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Differential expression of protein S genes during Myxococcus xanthus development.
J Bacteriol. 1985 Mar;161(3):1146-55. doi: 10.1128/jb.161.3.1146-1155.1985.
6
Localization of the cis-acting regulatory DNA sequences of the Myxococcus xanthus tps and ops genes.粘球菌tps和ops基因顺式作用调控DNA序列的定位
J Bacteriol. 1988 Oct;170(10):4931-8. doi: 10.1128/jb.170.10.4931-4938.1988.
7
Control of developmental gene expression by cell-to-cell interactions in Myxococcus xanthus.黄色黏球菌中细胞间相互作用对发育基因表达的调控。
J Bacteriol. 1986 Oct;168(1):341-7. doi: 10.1128/jb.168.1.341-347.1986.
8
Control of multicellular development: Dictyostelium and Myxococcus.多细胞发育的调控：盘基网柄菌和粘球菌。
Annu Rev Genet. 1986;20:539-66. doi: 10.1146/annurev.ge.20.120186.002543.
9
Intercellular signaling is required for developmental gene expression in Myxococcus xanthus.细胞间信号传导是黄色黏球菌发育基因表达所必需的。
Dev Biol. 1986 Sep;117(1):267-76. doi: 10.1016/0012-1606(86)90369-6.
10
Physical characterization of the genome of the Myxococcus xanthus bacteriophage MX-8.黄色粘球菌噬菌体MX-8基因组的物理特性
Mol Gen Genet. 1985;199(1):123-32. doi: 10.1007/BF00327521.