Department of Chemistry and Environmental Science, Zhangzhou Normal University, Zhangzhou, China.
J Agric Food Chem. 2011 Feb 9;59(3):822-8. doi: 10.1021/jf103480y. Epub 2011 Jan 5.
A new biomimetic digestion and absorption system, including in vitro bionic digestion and biomimetic membrane extraction, was used for the first time for the pretreatment of edible Laminaria japonica . After bionic digestion, 11 species of trace metals (V, Cr, Mn, Fe, Ni, Cu, Zn, Se, As, Cd, and Pb) in the resulting chyme were transformed into their final coordinated complexes and then absorbed by the biomembrane. Similar to the biomembrane between gastrointestinal tract and blood vessels, monolayer liposome was used for the first time as a biomembrane model. Affinity-monolayer liposome metals (AMLMs) were separated, determined by ICP-MS, and then used for the metal bioavailability assessment as the bioassimilated part. The action of gastrointestinal acidity and components (including digestive enzymes) was assessed on the basis of the concentration of AMLMs; the safe dosage and tolerable upper intake level of L. japonica for adults were proposed as 33.3 and 230.8 g/day, respectively.
首次采用新型仿生消化吸收系统，包括体外仿生消化和仿生膜提取，对食用昆布进行预处理。仿生消化后，食糜中 11 种痕量金属（V、Cr、Mn、Fe、Ni、Cu、Zn、Se、As、Cd 和 Pb）转化为其最终的配位络合物，然后被生物膜吸收。单层脂质体首次被用作仿生膜模型，类似于胃肠道和血管之间的生物膜。通过 ICP-MS 分离和测定亲和单层脂质体金属（AMLMs），然后将其作为可生物同化部分用于评估金属生物利用度。根据 AMLMs 的浓度评估了胃肠道酸度和成分（包括消化酶）的作用；提出成年人食用昆布的安全剂量和可耐受最高摄入量分别为 33.3 和 230.8 g/天。
