Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143-0738, USA.
Stem Cells Dev. 2011 Oct;20(10):1747-52. doi: 10.1089/scd.2010.0564. Epub 2011 Feb 24.
Stro-1 is the best-known mesenchymal stem cell marker. However, despite its bone marrow origin, its localization in bone marrow has never been demonstrated. By immunofluorescence staining, we show here that ∼ 0.74% of nucleated bone marrow cells expressed Stro-1. We also found that ∼ 8.7% of CD34-expressing cells expressed Stro-1, and more than 20% of Stro-1-expressing cells did not express CD34. In adipose tissue Stro-1 expression was identified in the endothelium of arterioles and capillaries. Stro-1 was also localized in the endothelium of some but not all adipose tissue veins. Endothelial expression of Stro-1 was also identified in blood vessels in penis and in leg muscles, but not in other tested tissues. In these other tissues, Stro-1 was scantly expressed near but not in blood vessels. These variable and endothelial expression patterns of Stro-1 point to a need to re-examine published data that relied on Stro-1 as a mesenchymal stem cell marker.
Stro-1 是最著名的间充质干细胞标志物。然而，尽管它起源于骨髓，但从未在骨髓中证明其定位。通过免疫荧光染色，我们在这里显示约 0.74%有核骨髓细胞表达 Stro-1。我们还发现，约 8.7%表达 CD34 的细胞表达 Stro-1，并且超过 20%表达 Stro-1 的细胞不表达 CD34。在脂肪组织中，Stro-1 被鉴定为小动脉和毛细血管的内皮细胞。Stro-1 也定位于一些但不是所有脂肪组织静脉的内皮细胞中。Stro-1 的内皮细胞表达也在阴茎和腿部肌肉的血管中被鉴定出来，但在其他测试组织中没有。在这些其他组织中，Stro-1 仅在血管附近表达，而不在血管中表达。Stro-1 的这种可变和内皮细胞表达模式表明需要重新检查依赖 Stro-1 作为间充质干细胞标志物的已发表数据。
