南洋杉死皮提取物及分离化合物的抗氧化活性和低细胞毒性。
Antioxidant activity and low cytotoxicity of extracts and isolated compounds from Araucaria angustifolia dead bark.
机构信息
Departamento de Ciências Farmacêuticas, CCS, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
出版信息
Redox Rep. 2010;15(6):234-42. doi: 10.1179/135100010X12826446921789.
The antioxidant activities of the extract and its relevant fraction as well as isolated compounds from the dead bark of Araucaria angustifolia are presented. This tree represents the Mixed Ombrophile Forest, which is endangered due to extensive logging. The dead bark of Araucaria is naturally discarded by the tree, and its hydroalcoholic crude extract has exhibited protective qualities against stress induced by H(2)O(2) in cell culture. Using several in vitro models, here we describe the antioxidant potential of the crude extract and its component ethyl acetate fraction and also of some compounds isolated from the ethyl acetate fraction. We provide the first description of the isolation of two natural product afzelechin derivatives. The extract and isolated compounds, particularly epiafzelechin protocatechuate (5), displayed very high antioxidant activity, as did compound 4, quercetin, which is well known for its antioxidant properties. In a DPPH assay, compound 5 exhibited an IC(50) of 0.7 μM; in a lipid peroxidation assay; IC(50) values of 21 μM and 35 μM were obtained when the oxidation was induced by UV and ascorbate free radicals, respectively.
介绍了来自南洋杉死皮的提取物及其相关部分以及分离化合物的抗氧化活性。这种树代表了混合湿生林，由于广泛的采伐，这种树濒临灭绝。南洋杉的死皮是由树木自然丢弃的，其水醇粗提取物在细胞培养中显示出对 H（2）O（2）诱导的应激的保护特性。使用几种体外模型，我们在这里描述了粗提取物及其成分乙酸乙酯部分以及从乙酸乙酯部分分离的一些化合物的抗氧化潜力。我们首次描述了两种天然产物穗花杉双黄酮衍生物的分离。提取物和分离的化合物，特别是表儿茶素原儿茶酸酯（5），表现出非常高的抗氧化活性，化合物 4，槲皮素也是如此，槲皮素因其抗氧化特性而广为人知。在 DPPH 测定中，化合物 5 的 IC（50）为 0.7 μM；在脂质过氧化测定中；当氧化分别由 UV 和抗坏血酸自由基诱导时，IC（50）值分别为 21 μM 和 35 μM。
Zotero 插件