Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN Quadra 916, Módulo B, Av. W5 Norte 70. 790-160 Asa Norte, Brasília, DF-Brazil.
Protein J. 2011 Jan;30(1):32-8. doi: 10.1007/s10930-010-9299-4.
Heavy agricultural losses are closely related to attacks by insect-pests and phytopathogens such as bacteria and fungi. Among them, the fungus Botrytis cinerea can cause gray mold in more than 200 different species of plants, and is considered a challenging problem for agribusiness. Fungicides are commonly used to control this pathogen because they are fast-working and easy to apply. However, the continuous use of fungicides may promote the selection of resistant fungi and can also cause profound contamination in ecosystems. Aiming to find alternative strategies to solve these problems, several studies have focused on searching for plant proteins and peptides with antifungal activities (AFPs). With this in mind, this report shows the isolation and characterization of two novels antifungal proteins from flowers of rosemary pepper (Lippia sidoides Cham.) with 10 and 15 kDa. Isolation was performed by using an Octyl-Sepharose hydrophobic column. In vitro bioassays indicated that isolated proteins were able to inhibit B. cinerea development, but were not effective against all bacteria tested. Moreover, N-termini sequences indicate that both proteins showed sequence homology with NBS-LRR R proteins with a lower molecular mass, suggesting possible protein fragmentation. Data reported here could help in the development of biotechnological products for crop protection against phytopathogenic fungi in the near future.
农业的重大损失与昆虫和植物病原菌（如细菌和真菌）的侵害密切相关。其中，灰葡萄孢菌可以引起 200 多种不同植物的灰霉病，是农业企业面临的一个具有挑战性的问题。杀菌剂通常用于控制这种病原体，因为它们作用迅速且易于使用。然而，杀菌剂的持续使用可能会促进抗真菌菌的选择，也会对生态系统造成严重污染。为了寻找替代策略来解决这些问题，一些研究集中在寻找具有抗真菌活性（AFPs）的植物蛋白和肽上。有鉴于此，本报告展示了从迷迭香椒（Lippia sidoides Cham.）花中分离和鉴定出两种新型抗真菌蛋白的研究结果，这两种蛋白的分子量分别为 10 kDa 和 15 kDa。分离是通过使用辛基琼脂糖疏水柱进行的。体外生物测定表明，分离出的蛋白能够抑制灰葡萄孢菌的生长，但对所有测试的细菌都没有效果。此外，N 末端序列表明这两种蛋白与 NBS-LRR R 蛋白具有序列同源性，但其分子量较低，表明可能发生了蛋白片段化。本报告的数据有助于在不久的将来开发生物技术产品，以保护作物免受植物病原菌的侵害。
