Rajgopal Arun, Rebhun John F, Burns Charlie R, Scholten Jeffrey D, Balles John A, Fast David J
1 Analytical Sciences, Amway Corporation , Ada, Michigan, USA .
J Med Food. 2015 Mar;18(3):370-7. doi: 10.1089/jmf.2014.0096. Epub 2015 Jan 19.
Lippia sidoides is an aromatic shrub that grows wild in the northeastern region of Brazil. In local traditional medicine, the aerial portions of this species are used as anti-infectives, antiseptics, spasmolytics, sedatives, hypotensives, and anti-inflammatory agents. In this research, we evaluate the potential immunological properties of Lippia extract through in vitro analysis of its ability to modulate intracellular cyclic adenosine monophosphate (cAMP) levels and interleukin-10 (IL-10) production. These results show that Lippia extract increases intracellular cAMP through the inhibition of phosphodiesterase activity. They also demonstrate that Lippia extract increases IL-10 production in THP-1 monocytes through both an increase in intracellular cAMP and the activation of p38 MAPK. These results suggest that the Lippia-mediated inhibition of phosphodiesterase activity and the subsequent increase in intracellular cAMP may explain some of the biological activities associated with L. sidoides. In addition, the anti-inflammatory activity of L. sidoides may also be due, in part, to its ability to induce IL-10 production through the inhibition of cyclic nucleotide-dependent phosphodiesterase activity and by its activation of the p38 MAPK pathway.
拟香茶菜是一种野生于巴西东北部地区的芳香灌木。在当地传统医学中，该物种的地上部分被用作抗感染药、防腐剂、解痉剂、镇静剂、降压药和抗炎药。在本研究中，我们通过体外分析拟香茶菜提取物调节细胞内环磷酸腺苷（cAMP）水平和白细胞介素-10（IL-10）产生的能力，来评估其潜在的免疫学特性。这些结果表明，拟香茶菜提取物通过抑制磷酸二酯酶活性来增加细胞内cAMP。它们还证明，拟香茶菜提取物通过增加细胞内cAMP和激活p38丝裂原活化蛋白激酶（p38 MAPK），来增加THP-1单核细胞中IL-10的产生。这些结果表明，拟香茶菜介导的磷酸二酯酶活性抑制以及随后细胞内cAMP的增加，可能解释了与拟香茶菜相关的一些生物学活性。此外，拟香茶菜的抗炎活性也可能部分归因于其通过抑制环核苷酸依赖性磷酸二酯酶活性和激活p38 MAPK途径来诱导IL-10产生的能力。
