[细胞色素P450 1A1基因多态性、谷胱甘肽S-转移酶M1缺失、烹饪油烟与非吸烟女性肺癌风险]

[CYP1A1 polymorphisms, lack of glutathione S-transferase M1 (GSTM1), cooking oil fumes and lung cancer risk in non-smoking women].

作者信息

Zhu Xiao-Xia, Hu Cheng-Ping, Gu Qi-Hua

机构信息

Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2010 Nov;33(11):817-22.

PMID:21211368

Abstract

OBJECTIVE

to study the correlation of polymorphisms of CYP1A1 MspI, GSTM1 null genotype, cooking oil fumes independently and in combination with the risk of non-smoking lung cancer in females.

METHODS

one hundred and sixty female non-smoking patients with primary lung cancer and 160 controls were enrolled from Xiangya Hospital of Central South University. PCR-RELP and PCR were used to detect the distribution of CYP1A1 MspI and GSTM1 genotypes respectively. The correlation of these genes and cooking oil fumes with the susceptibility to lung cancer was analyzed.

RESULTS

There was a significant difference in the frequencies of cooking oil fumes exposure between cancer cases and controls (χ(2) = 10.734, P < 0.01);but there was no statistical difference in CYP1A1 MspI polymorphisms between the 2 groups (χ(2) = 3.731, P > 0.05). The combination of CYP1A1 polymorphisms and cooking oil fumes significantly increased the risk of lung cancer. The frequencies of GSTM1 null genotype was significantly different between cancer cases and controls (χ(2) = 0.518, P < 0.05). The risk of lung cancer was higher in those with the GSTM1 null genotype and the OR was 1.697 (95%CI 1.090 - 2.640). Individuals with both GSTM1 null genotype and exposure to cooking fumes had a higher risk of cancer than those with only one of them, the OR being 3.617 (95%CI 1.899 - 6.891). The combination of the two genes significantly increased the risk of lung cancer.

CONCLUSIONS

cooking oil fumes exposure was a risk factor for non-smoking lung cancer in females. The combination of CYP1A1 with cooking oil fume increased the risk of female lung cancer. GSTM1 null genotype was associated with risk of lung cancer in non-smoking females. The combination of GSTM1 null genotype and cooking oil fumes significantly increased the risk of female lung cancer. The combination of CYP1A1 and GSTM1 significantly increased the risk of lung cancer.

摘要

目的

研究细胞色素P450 1A1（CYP1A1）MspI多态性、谷胱甘肽S转移酶M1（GSTM1）基因缺失型分别及联合食用油油烟暴露与女性非吸烟肺癌风险的相关性。

方法

选取中南大学湘雅医院160例女性原发性非吸烟肺癌患者和160例对照。分别采用聚合酶链反应-限制性片段长度多态性（PCR-RELP）和聚合酶链反应（PCR）检测CYP1A1 MspI和GSTM1基因的分布。分析这些基因及食用油油烟暴露与肺癌易感性的相关性。

结果

病例组与对照组食用油油烟暴露频率差异有统计学意义（χ(2)=10.734，P<0.01）；但两组间CYP1A1 MspI多态性差异无统计学意义（χ(2)=3.731，P>0.05）。CYP1A1多态性与食用油油烟暴露联合作用显著增加肺癌风险。病例组与对照组GSTM1基因缺失型频率差异有统计学意义（χ(2)=0.518，P<0.05）。GSTM1基因缺失型个体患肺癌风险较高，比值比（OR）为1.697（95%可信区间1.090 - 2.640）。同时具有GSTM1基因缺失型和食用油油烟暴露的个体患癌风险高于仅有一种情况的个体，OR为3.617（95%可信区间1.899 - 6.891）。两种基因联合作用显著增加肺癌风险。