Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. Coruña Km. 7.5, 28040 Madrid, Spain.
Vaccine. 2011 Feb 17;29(9):1830-5. doi: 10.1016/j.vaccine.2010.12.081. Epub 2011 Jan 4.
In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infectivity in cell culture and in suckling mice. All vaccinated animals were fully protected when challenged with neurovirulent WNV NY99. Passive transfer of protective antibodies from immunized mothers to their offspring occurred both by transplacental and lactation routes. These results indicate that the insect-derived antigens tested may constitute potential vaccine candidates to be further evaluated.
在这项研究中，西尼罗河病毒（WNV）包膜（rE）蛋白及其结构域 III（rDIII）在基于昆虫幼虫的经济高效系统中得到有效表达，这些幼虫是非发酵性的活体生物工厂。用部分纯化的 rE 或 rDIII 免疫的小鼠诱导出高抗体滴度，可中和细胞培养物中和幼鼠中的病毒感染性。所有接种疫苗的动物在受到神经毒力 WNV NY99 的攻击时均受到完全保护。来自免疫母亲的保护性抗体通过胎盘和哺乳途径被动转移到其后代。这些结果表明，所测试的昆虫衍生抗原可能构成潜在的疫苗候选物，有待进一步评估。
