Nankai University, Tianjin 300071, P. R. China.
Am J Chin Med. 2011;39(1):111-20. doi: 10.1142/S0192415X11008683.
Ischemic stroke is one of the most common causes of death and disability that is induced by ischemia reperfusion (IR). Granulocyte adherence has been proven to be a principal cause of IR. Salvianolic acid A (Sal A) is one of the major active components of Danshen, a Chinese herbal medicine used for the treatment of cardiovascular and cerebrovascular diseases, such as ischemic stroke. Some experimental studies have shown the strong cerebral protection effect of Sal A. However, little information is available about the effect of Sal A on granulocyte adherence to brain micro-vascular endothelial cells (BMEC). Therefore, the aim of the present study was to investigate the effect of Sal A on the leukocyte adhesion rate and the intercellular cell adhesion molecule-1 (ICAM-1) expression in BMEC injured by hypoxia/reoxygenation (H/R), using a rheometer, qRT-PCR, and flow cytometry (FCM). The results of the adhesion rate gathered by the rheometer showed that Sal A could remarkably inhibit the adherence of granulocytes on BMEC in the case of H/R injury. Moreover, PCR and FCM results showed that Sal A could decrease the expression of ICAM-1 on BMEC on the gene and protein levels. In conclusion, the study demonstrated that the inhibition of granulocyte adherence is one of the targets of Sal A in the treatment of ischemic stroke. Meanwhile, Sal A inhibits of granulocyte adherence by decreasing the expression of ICAM-1 in BMEC.
缺血性中风是由缺血再灌注（IR）引起的最常见的死亡和残疾原因之一。粒细胞黏附已被证明是 IR 的主要原因之一。丹酚酸 A（Sal A）是丹参的主要活性成分之一，丹参是一种用于治疗心血管和脑血管疾病的中药，如缺血性中风。一些实验研究表明 Sal A 具有很强的脑保护作用。然而，关于 Sal A 对缺氧/复氧（H/R）损伤的脑微血管内皮细胞（BMEC）中白细胞黏附的影响的信息很少。因此，本研究旨在使用流变仪、qRT-PCR 和流式细胞术（FCM）研究 Sal A 对 H/R 损伤的 BMEC 中白细胞黏附率和细胞间黏附分子-1（ICAM-1）表达的影响。流变仪采集的黏附率结果表明，Sal A 可显著抑制 H/R 损伤时粒细胞与 BMEC 的黏附。此外，PCR 和 FCM 结果表明 Sal A 可降低 BMEC 上 ICAM-1 的基因和蛋白水平表达。综上所述，该研究表明抑制粒细胞黏附是 Sal A 治疗缺血性中风的靶点之一。同时，Sal A 通过降低 BMEC 中 ICAM-1 的表达来抑制粒细胞黏附。
