Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, South Korea.
Bioresour Technol. 2011 Mar;102(5):4277-80. doi: 10.1016/j.biortech.2010.12.039. Epub 2010 Dec 22.
The optimum conditions for the production of L-arabinose from debranched arabinan were determined to be pH 6.5, 75°C, 20 g l(-1) debranched arabinan, 42 Um l(-1) endo-1,5-α-L-arabinanase, and 14 U ml(-1) α-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus and the conditions for sugar beet arabinan were pH 6.0, 75°C, 20 g l(-1) sugar beet arabinan, 3 U ml(-1) endo-1,5-α-L-arabinanase, and 24 U ml(-1) α-L-arabinofuranosidase. Under the optimum conditions, 16 g l(-1)l-arabinose was obtained from 20 g l(-1) debranched arabinan or sugar beet arabinan after 120 min, with a hydrolysis yield of 80% and a productivity of 8 g l(-1)h(-1). This is the first reported trial for the production of L-arabinose from the hemicellulose arabinan by the combined use of endo- and exo-arabinanases.
最佳条件下的生产 L-阿拉伯糖从支链阿拉伯聚糖被确定为 pH 值 6.5，75°C，20 g l(-1)去支化阿拉伯聚糖，42 Um l(-1)内切 1,5-α-L-阿拉伯聚糖酶和 14 U ml(-1)α-L-阿拉伯呋喃糖苷酶从 Caldicellulosiruptor saccharolyticus 和条件下的糖甜菜阿拉伯聚糖 pH 值 6.0，75°C，20 g l(-1)糖甜菜阿拉伯聚糖，3 U ml(-1)内切 1,5-α-L-阿拉伯聚糖酶和 24 U ml(-1)α-L-阿拉伯呋喃糖苷酶。在最佳条件下，16 g l(-1)l-阿拉伯糖从 20 g l(-1)去支化阿拉伯聚糖或糖甜菜阿拉伯聚糖后 120 分钟，水解率为 80%和生产力 8 g l(-1)h(-1)。这是第一次报道试验生产 L-阿拉伯糖的半纤维素阿拉伯聚糖联合使用内切和外切阿拉伯聚糖酶。
