Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.
Bioresour Technol. 2010 Nov;101(21):8300-7. doi: 10.1016/j.biortech.2010.05.070. Epub 2010 Jun 20.
The filamentous fungus Chrysosporium lucknowense (C1) is a rich source of cell wall degrading enzymes. In the present paper four arabinose releasing enzymes from C1 were characterized, among them one endoarabinanase, two arabinofuranosidases and one exoarabinanase. Combinations of these enzymes released up to 80% of the arabinose present in sugar beet arabinan to fermentable monosugars. Besides the main product arabinobiose, unknown arabinose oligomers are produced from highly branched arabinan when endoarabinanase was combined with exoarabinanase and/or arabinofuranosidase. All described arabinose releasing enzymes are temperature stable up to 50 degrees C and have a broad pH stability. This makes C1 arabinohydrolases suitable for many biotechnical applications, like co-fermentation bioethanol production.
丝状真菌 Chrysosporium lucknowense（C1）是细胞壁降解酶的丰富来源。在本文中，我们对 C1 中的四种阿拉伯糖释放酶进行了表征，其中包括一种内切阿拉伯聚糖酶、两种阿拉伯呋喃糖苷酶和一种外切阿拉伯聚糖酶。这些酶的组合可以将糖甜菜阿拉伯聚糖中高达 80%的阿拉伯糖释放为可发酵的单糖。当内切阿拉伯聚糖酶与外切阿拉伯聚糖酶和/或阿拉伯呋喃糖苷酶组合使用时，除了主要产物阿拉伯二糖外，还会从高度支化的阿拉伯聚糖中产生未知的阿拉伯糖低聚物。描述的所有阿拉伯糖释放酶在 50°C 以下温度稳定，并且具有广泛的 pH 稳定性。这使得 C1 阿拉伯水解酶适用于许多生物技术应用，例如共发酵生物乙醇生产。
