Bartošíková Lenka, Nečas Jiří, Bartošík Tomáš, Pavlík Martin, Fráňa Petr
Department of Physiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
Interdiscip Toxicol. 2010 Jun;3(2):76-81. doi: 10.2478/v10102-010-0015-1.
The aim of the study was to analyse protective effects of different doses of pomiferin in therapy of reperfusion injury. Rats were randomly divided into five groups (n=10). One group was intact. Three medicated groups and one placebo group were subjected to ischaemia and reperfusion of the left kidney. Pomiferin was administrated by single gastric gavage in 2 ml of 0.5% Avicel solution in doses of 5, 10 and 20 mg/kg. The placebo group was given only Avicel solution. On day 15, all the animals were exsanguinated and the reperfused kidneys were recovered. Selected biochemical markers were assessed in blood: antioxidative enzymes, total antioxidative capacity, malondialdehyde, creatinine, urea and uric acid. Creatinine, urea and total proteins were analysed in urine and 24-hour diuresis was recorded. The kidney tissue samples were used for histopathological examination.The results confirmed the expected protective effects of pomiferin. Pomiferin supported defensive reactions of the body against free radicals (increased levels of superoxide dismutase, total antioxidative capacity), decreased lipid peroxidation (decreased malondialdehyde) and contributed to the recovery of kidney functions (creatinine and urea in blood). The best biochemical and histopathological results were achieved after pomiferin administration in the dose of 5 mg/kg.
本研究的目的是分析不同剂量的柚皮素在再灌注损伤治疗中的保护作用。将大鼠随机分为五组（n = 10）。一组为完整组。三个用药组和一个安慰剂组接受左肾缺血再灌注。柚皮素以5、10和20 mg/kg的剂量通过单次灌胃给予2 ml 0.5%的微晶纤维素溶液。安慰剂组仅给予微晶纤维素溶液。在第15天，所有动物放血处死，取出再灌注的肾脏。检测血液中选定的生化指标：抗氧化酶、总抗氧化能力、丙二醛、肌酐、尿素和尿酸。分析尿液中的肌酐、尿素和总蛋白，并记录24小时尿量。肾脏组织样本用于组织病理学检查。结果证实了柚皮素预期的保护作用。柚皮素支持机体对自由基的防御反应（超氧化物歧化酶水平升高、总抗氧化能力增强），降低脂质过氧化（丙二醛降低），并有助于肾功能的恢复（血液中的肌酐和尿素）。给予5 mg/kg剂量的柚皮素后，获得了最佳的生化和组织病理学结果。
