Fisher E R, Paulson J D
Cancer Res. 1978 Nov;38(11 Pt 1):3830-5.
A new tissue culture cell line, SHP-77, has been established from explant cultures of a primary human lung cancer. Although the latter exhibited histological features of the so-called large or polygonal cell undifferentiated variant, neurosecretory granules were detected ultrastructurally in cells of the original tumor, culture line, and neoplasms developing after transplantation of the latter in nude mice. These features attest to the identity of the original tumor as a large-cell variant of oat cell cancer. In addition, electron microscopy revealed the presence of gland formation and intracytoplasmic lamellar bodies in the cells of SHP-77. This information indicates the potential for varied differentiation and morphological expression of so-called undifferentiated lung cancer cells and is pertinent to nosological considerations concerning human lung cancer. The cell line has maintained its morphological, karyotypic, chromosomal, and growth characteristics after transplantation to nude mice. Because of this stability, SHP-77 appears to represent a propitious cell line for in vitro and in vivo biological and therapeutic studies of this type of lung cancer.
一种新的组织培养细胞系SHP - 77已从原发性人肺癌的外植体培养物中建立。尽管后者表现出所谓大细胞或多边形细胞未分化变体的组织学特征，但在原始肿瘤、培养细胞系以及将后者移植到裸鼠后形成的肿瘤细胞中，通过超微结构检测到了神经分泌颗粒。这些特征证明原始肿瘤是燕麦细胞癌的大细胞变体。此外，电子显微镜显示SHP - 77细胞中存在腺体形成和胞质内板层小体。这一信息表明所谓未分化肺癌细胞具有多种分化和形态学表达的潜力，并且与人类肺癌的疾病分类学考虑相关。该细胞系在移植到裸鼠后保持了其形态、核型、染色体和生长特征。由于这种稳定性，SHP - 77似乎是用于此类肺癌体外和体内生物学及治疗研究的理想细胞系。
