Lai B T
Beijing Lung Tumor Research Institute.
Zhonghua Zhong Liu Za Zhi. 1988 Jul;10(4):253-5.
A serially transplatable tumor model was established by inoculating subcutaneously the surgical specimen of a patient with mixed type small cell lung carcinoma into nude mice (BALB/C nu/nu). A human small cell lung carcinoma cell line cultured in vitro was established from that tumor in nude mice. The cultured cell line gave a typical growth pattern with small round feature, cell clusters in suspension and float growth. Pathological sections of both the patient's tumor and transplantable tumor in nude mice showed the same morphological features. The typical APUD granules were observed in electron transmission microscopy. The transplantable tumor has been transferred for 9 passages in 14 months and the cell line in vitro for 71 passages in nearly one year.
通过将一名混合型小细胞肺癌患者的手术标本皮下接种到裸鼠（BALB/C nu/nu）中，建立了一种可连续传代移植的肿瘤模型。从裸鼠体内的该肿瘤中建立了一种体外培养的人小细胞肺癌细胞系。培养的细胞系呈现出典型的生长模式，具有小圆形特征、悬浮细胞簇和漂浮生长。患者肿瘤和裸鼠体内可移植肿瘤的病理切片显示出相同的形态学特征。在电子透射显微镜下观察到典型的APUD颗粒。可移植肿瘤在14个月内传代了9次，体外细胞系在近一年内传代了71次。
