Koros A M, Klein E C, Pan S, Atchison R W, Lakomy R, Bahnson A, Sherer C
Cancer Res. 1985 Jun;45(6):2725-31.
The human small cell (oat cell) carcinoma line, SHP-77, established by Fisher and Paulson in 1977 and originally described as a "large cell variant of oat cell cancer" has been evaluated by several different parameters and shown even after more than 200 passages to retain properties described for the original cell line. Karyotypic, histological, and biochemical features are retained, as well as tumorigenicity in nude mice. The original authors' suggestion that this is a propitious cell line for both in vitro and in vivo studies is supported by this report. Modulation of growth characteristics in vivo (in xenografts) emphasizes the plasticity of this unique line which serves as a valuable model for basic as well as therapeutic studies. SHP-77 can serve as an in vitro target in 51Cr and 111In release cytotoxicity assays as well as in in vivo nude mouse assays for evaluating immune reactivity of cells and serum from lung cancer patients. The potential histological variability of SHP-77, despite its biochemical stability, calls attention to the inadequacy of histological criteria for lung tumor classification.
人小细胞（燕麦细胞）癌系SHP - 77由费舍尔和保尔森于1977年建立，最初被描述为“燕麦细胞癌的大细胞变体”。通过几个不同参数对其进行了评估，结果显示，即使经过200多次传代，它仍保留了原细胞系所描述的特性。核型、组织学和生化特征得以保留，在裸鼠中的致瘤性也得以保留。本报告支持了原作者的观点，即这是一种适合体外和体内研究的理想细胞系。体内（异种移植）生长特性的调节强调了这一独特细胞系的可塑性，它是基础研究和治疗研究的宝贵模型。SHP - 77可作为51Cr和111In释放细胞毒性试验的体外靶标，也可用于体内裸鼠试验，以评估肺癌患者细胞和血清的免疫反应性。尽管SHP - 77具有生化稳定性，但其潜在的组织学变异性提醒人们注意肺肿瘤分类中组织学标准的不足。
