Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210097, Jiangsu, China.
J Ethnopharmacol. 2011 Apr 12;134(3):624-9. doi: 10.1016/j.jep.2011.01.003. Epub 2011 Jan 8.
To investigate the immunological regulation of Guizhi Fuling Capsule (GZFLC) on rat endometriosis.
Twenty-seven rats, in which endometriotic implants were induced by transplanting autologous uterine tissue to the peritoneum, were randomly divided into three groups equally: (1) the GZFLC group of low dose (480 mg/kg/day); (2) the GZFLC group of high dose (1,920 mg/kg/day); and (3) the model group(saline solution). Another 10 rats were treated as sham operation group. After rats were treated for four weeks, we examined the alterations of implants volume, the percentage of CD4(+) T lympholeukocyte, the activity of NK cell and the expression of cytokines (MCP-1 and ICAM-1) on each group.
Statistical analysis showed that posttreatment volumes were significantly reduced compared with pretreatment in GZFLC groups, whereas there was no significant change in the model group. The percentage of CD4(+) T lympholeukocyte and the activity of NK cell in GZFLC groups significantly increased to the level of the sham group compared with the model. RT-PCR and immunohistochemistry showed that the endometria of the sham operation and treatment groups were similar on expression level of MCP-1 and ICAM-1.
GZFLC plays an important role in the regression of endometriotic implants by immunological regulation in the rat model.
研究桂枝茯苓胶囊（GZFLC）对大鼠子宫内膜异位症的免疫调节作用。
将 27 只大鼠的自体子宫组织移植到腹腔中诱导子宫内膜异位症，然后将它们随机均分为 3 组：（1）GZFLC 低剂量组（480mg/kg/天）；（2）GZFLC 高剂量组（1920mg/kg/天）；（3）模型组（生理盐水）。另 10 只大鼠作为假手术组。大鼠治疗 4 周后，我们检查了各组植入物体积、CD4+T 淋巴细胞百分比、NK 细胞活性和细胞因子（MCP-1 和 ICAM-1）的表达变化。
统计分析显示，与治疗前相比，GZFLC 组治疗后体积明显减小，而模型组则没有明显变化。与模型组相比，GZFLC 组 CD4+T 淋巴细胞百分比和 NK 细胞活性显著增加，达到假手术组水平。RT-PCR 和免疫组化显示，假手术组和治疗组的 MCP-1 和 ICAM-1 表达水平相似。
GZFLC 通过免疫调节在大鼠模型中对子宫内膜异位症植入物的消退起着重要作用。
