Andersson N, Morales A, Nava E, Martinez E, Rodriguez I, Young P, Howard M K, Miles M A
Centre for Tropical Disease Research, Facultad de Medicina, Universidad Autónoma de Guerrero, Acapulco, Mexico.
J Trop Med Hyg. 1990 Oct;93(5):341-6.
The enzyme linked immunosorbent assay (ELISA) was used to analyse 4372 blood samples from residents of 978 households in 20 representative communities in the Mexican state of Guerrero. Seventy-five individuals had very high titres of antibodies against Trypanosoma cruzi. Samples with intermediate optical density values, despite overlapping values with several control positives on a single-well test, did not sustain their positivity at high dilutions. 'Intermediate positives' had a different distribution among the 20 communities to samples sustaining reactivity at high dilutions, indicating possible cross-reactivity with another infectious agent. The finding of seropositive children under the age of 10 years in the Costa Chica, Acapulco and the Tierra Caliente regions, with family clustering of putative cases, indicates that recent transmission must be considered. Very few people interviewed in the 20 communities knew the triatomine bug could transmit a disease.
采用酶联免疫吸附测定（ELISA）对墨西哥格雷罗州20个代表性社区中978户家庭的4372份血液样本进行了分析。75人针对克氏锥虫的抗体滴度非常高。尽管在单孔检测中，光密度值处于中间水平的样本与多个对照阳性样本的值有重叠，但在高稀释度下其阳性反应无法维持。“中间阳性”样本在20个社区中的分布与在高稀释度下仍保持反应性的样本不同，这表明可能与另一种感染因子存在交叉反应。在科斯塔奇卡、阿卡普尔科和蒂拉卡连特地区发现10岁以下血清阳性儿童，且疑似病例有家庭聚集现象，这表明必须考虑近期存在传播情况。在这20个社区接受采访的人中，很少有人知道锥蝽能够传播疾病。
