Akaĭzin E S, Voskun S E, Panova L A, Smirnov S G
Mikrobiologiia. 1990 Mar-Apr;59(2):283-8.
Escherichia coli M-17 autolysis was induced by eliminating nutrition sources from the growth medium and exerting a shock with EDTA. The overall cell number, the optical density of the cell suspension, the number of colony-forming units (CFU), and [3H]uracil incorporation into the cells were analysed in the course of autolysis. The number of CFU was found to drop down faster than the overall cell number in the process of autolysis. The population of E. coli was shown to be heterogeneous in its sensitivity to the induction of autolysis, and some nonlysed cells were still metabolically active. When the rate of autolysis was highest in some cells of the population, the labeled precursor was found to be incorporated into the TCA-soluble and TCA-insoluble fractions of nonlysed cells. The overall cell number, the optical density of the cell suspension, and the number of CFU increased 96 h after the induction of autolysis. The authors discuss what is the role played by the heterogeneity of an E. coli population in its adaptation to EDTA-induced autolysis.
通过从生长培养基中去除营养源并用乙二胺四乙酸（EDTA）施加冲击来诱导大肠杆菌M - 17自溶。在自溶过程中分析了总细胞数、细胞悬液的光密度、菌落形成单位（CFU）数量以及[³H]尿嘧啶掺入细胞的情况。发现在自溶过程中CFU数量的下降速度比总细胞数更快。结果表明，大肠杆菌群体对自溶诱导的敏感性存在异质性，并且一些未裂解的细胞仍具有代谢活性。当群体中的一些细胞自溶速率最高时，发现标记的前体被掺入到未裂解细胞的三羧酸循环（TCA）可溶性和TCA不溶性部分中。自溶诱导96小时后，总细胞数、细胞悬液的光密度和CFU数量增加。作者讨论了大肠杆菌群体的异质性在其适应EDTA诱导的自溶过程中所起的作用。
